Ephrin B1 Protein (EFNB1) (His tag)

Catalog No. ABIN7533927

Recombinant Ephrin B1 protein expressed in HEK-293 Cells.

Quick Overview for Ephrin B1 Protein (EFNB1) (His tag) (ABIN7533927)

Target: Ephrin B1 (EFNB1)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % by SDS-PAGE.

Product Details

Purification tag / Conjugate: This Ephrin B1 protein is labelled with His tag.

Purpose: Active Recombinant Human Ephrin-B1/EFNB1 Protein

Sequence: LAKNLEPVSW SSLNPKFLSG KGLVIYPKIG DKLDIICPRA EAGRPYEYYK LYLVRPEQAA ACSTVLDPNV LVTCNRPEQE IRFTIKFQEF SPNYMGLEFK KHHDYYITST SNGSLEGLEN REGGVCRTRT MKIIMKVGQD PNAVTPEQLT TSRPSKEADN TVKMATQAPG SRGSLGDSDG KHETVNQEEK SGPGASGGSS GDPDG

Specificity: Leu28-Gly232

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Human EFNB1 at 0.5 μg/mL (100 μL/well) can bind Mouse EPHB3 with a linear range of 0.1-3.5 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: Ephrin B1 (EFNB1)

Alternative Name: Ephrin-B1/EFNB1

Background:

Description: Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins.

Name: EFNB1,CFND,CFNS,EFB1,EFL3,EPLG2,Elk-L,LERK2,ephrin-B1

Gene ID: 1947

UniProt: P98172

Pathways: RTK Signaling