IDO1 Protein

Catalog No. ABIN7520080

This Recombinant IDO1 protein is produced in Escherichia coli (E. coli).

Quick Overview for IDO1 Protein (ABIN7520080)

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 97 % by SDS-PAGE.

Product Details

Purpose: Active Recombinant Human IDO-1 Protein

Sequence: AHAMENSWTI SKEYHIDEEV GFALPNPQEN LPDFYNDWMF IAKHLPDLIE SGQLRERVEK LNMLSIDHLT DHKSQRLARL VLGCITMAYV WGKGHGDVRK VLPRNIAVPY CQLSKKLELP PILVYADCVL ANWKKKDPNK PLTYENMDVL FSFRDGDCSK GFFLVSLLVE IAAASAIKVI PTVFKAMQMQ ERDTLLKALL EIASCLEKAL QVFHQIHDHV NPKAFFSVLR IYLSGWKGNP QLSDGLVYEG FWEDPKEFAG GSAGQSSVFQ CFDVLLGIQQ TAGGGHAAQF LQDMRRYMPP AHRNFLCSLE SNPSVREFVL SKGDAGLREA YDACVKALVS LRSYHLQIVT KYILIPASQQ PKENKTSEDP SKLEAKGTGG TDLMNFLKTV RSTTEKSLLK EG

Specificity: Ala2-Gly403

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 8.0.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: IDO1 (Indoleamine 2,3-Dioxygenase 1 (IDO1))

Alternative Name: IDO-1

Background:

Description: Indoleamine 2,3-dioxygenase (IDO) is a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.

Name: IDO, IDO-1, INDO,IDO1,IDO-1,INDO,indoleamine 2

Gene ID: 3620

UniProt: P14902

Pathways: Activated T Cell Proliferation