IGF1 Protein
Quick Overview for IGF1 Protein (ABIN7535993)
Target
See all IGF1 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purpose
- Active Recombinant Human IGF-I Protein
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Sequence
- GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA
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Specificity
- Gly49-Ala118
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- < 0.1 EU/μg of the protein by LAL method.
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Biological Activity Comment
- Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 53.5-214.2 ng/mL.
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Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) (Active) protein
IGF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Insulin-Like Growth Factor 1 (IGF1) (Active) proteinIGF1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Insulin-Like Growth Factor 1 (IGF1) (Active) proteinIGF1 Origin: Rat Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Insulin-Like Growth Factor 1 (IGF1) protein (Fc Tag)IGF1 Origin: Mouse Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Insulin-Like Growth Factor 1 (IGF1) protein (Fc Tag)IGF1 Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Insulin-Like Growth Factor 1 (IGF1) (AA 1-115) (Active) proteinIGF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS Active
Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) protein (His-IF2DI Tag)IGF1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Insulin-Like Growth Factor 1 (IGF1) (AA 49-118) (Active) protein (His tag)IGF1 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Insulin-Like Growth Factor 1 (IGF1) (AA 49-138) protein (GST tag)IGF1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
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Storage
- -20 °C,-80 °C
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Storage Comment
- Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
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- IGF1 (Insulin-Like Growth Factor 1 (IGF1))
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Alternative Name
- IGF-I
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Background
- IGF-1,IGF-I,IGF1A,IGFI,IGF?I,MGF,IGF1
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Gene ID
- 3479
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UniProt
- P05019-1
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Pathways
- RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation
Target
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