IGF1 Protein (AA 1-115)

Catalog No. ABIN934921

Product Details

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 1-115

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Sequence: MGPETLCGAE LVDALQFVCG DRGFYFNKPT GYGSSSRRAP QTGIVDECCF RSCDLRRLEM YCAPLKPAKS A

Characteristics: Purified recombinant Human IGF1 protein
Expression System: E.coli
Bioactivity: The ED50 for this effect is < 3.6 ng/mL. Measured in a cell proliferation assay using MCF7 cell.
Molecular weight on SDS-PAGE will appear higher.

Purity: > 95 % pure

Application Details

Application Notes: IGF1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Assay Procedure:

Cell line: MCF7 (human) Maintenance Condition: RPMI 1640 containing 10 % FBS Assay Medium: serum free RPMI 1640 Cell Density: 2 x 10,000 cells/well (96 well plate, final volume 100 µL) Serum Free Starvation: 24 h with RPMI1640 Incubation Time: 40 hr (after sample treatment) Concentration Range: 0.39 ng/mL - 29 ng/mL Detection Method: BrdU assay

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in PBS, pH 7.4.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: IGF1 (IGF1 Products)

Synonyms: IGF-I Protein, IGF1A Protein, IGFI Protein, C730016P09Rik Protein, Igf-1 Protein, Igf-I Protein, Npt2B Protein, IGF-1 Protein, IGFIA Protein, IGF-IB Protein, igf1 Protein, IGF-1L Protein, IGF-1a Protein, IGF1 Protein, igf-1 Protein, igf1-A Protein, igf1.S Protein, xigf1 Protein, insulin like growth factor 1 Protein, insulin-like growth factor 1 Protein, insulin-like growth factor I Protein, insulin like growth factor 1 L homeolog Protein, IGF1 Protein, Igf1 Protein, LOC100136741 Protein, igf1 Protein, LOC678666 Protein, igf1.L Protein, LOC104911603 Protein

Background: The Insulin-like growth factor-1(IGF-1) is a mitogenic polypeptide growth factors that stimulates the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGF-1 is predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGF-1 belongs to the insulin gene family, which also contains insulin and relaxin. The IGF-1s are similar in structure and function to insulin, but have a much higher growth-promoting activity than insulin. This recombinant human IGF-I is a globular protein containing 70 amino acids, and 3 intra-molecular disulfide bonds. Recombinant IGF-1 was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Alternative Names: MGF protein, IGF 1 protein, IGF-IA protein, Insulin like growth factor 1 protein, Insulin like growth factor 1 (somatomedin C) protein, Insulin like growth factor IB protein, IGF1 protein, IGFI protein, IGF IB protein, IGF1, IGF-1, IGF 1, IBP1 protein, IGF-1 protein, Insulin-like growth factor-1 protein, IGF1 protein, Mechano growth factor protein, IGF-1 protein, , Somatomedin C protein, IGFIA protein, Insulin like growth factor IA protein, Insulin-like growth factor-1 IGF IA protein, IBP1 protein, Somatomedin-C protein

Molecular Weight: 7.7 kDa (71 AA)

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation