Renin Protein (REN) (His tag)
Quick Overview for Renin Protein (REN) (His tag) (ABIN7534892)
Target
See all Renin (REN) ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purification tag / Conjugate
- This Renin protein is labelled with His tag.
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Purpose
- Active Recombinant Human Renin/REN Protein
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Sequence
- LPTDTTTFKR IFLKRMPSIR ESLKERGVDM ARLGPEWSQP MKRLTLGNTT SSVILTNYMD TQYYGEIGIG TPPQTFKVVF DTGSSNVWVP SSKCSRLYTA CVYHKLFDAS DSSSYKHNGT ELTLRYSTGT VSGFLSQDII TVGGITVTQM FGEVTEMPAL PFMLAEFDGV VGMGFIEQAI GRVTPIFDNI ISQGVLKEDV FSFYYNRDSE NSQSLGGQIV LGGSDPQHYE GNFHYINLIK TGVWQIQMKG VSVGSSTLLC EDGCLALVDT GASYISGSTS SIEKLMEALG AKKRLFDYVV KCNEGPTLPD ISFHLGGKEY TLTSADYVFQ ESYSSKKLCT LAIHAMDIPP PTGPTWALGA TFIRKFYTEF DRRNNRIGFA LAR
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Specificity
- Leu24-Arg406
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- < 0.1 EU/μg of the protein by LAL method.
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Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized Human REN Protein at 0.5 μg/mL (100 μL/well) can bind Renin Rabbit pAb with a linear range of 1.9-302.7 ng/mL.
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Renin (REN) protein (His tag)
REN Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Renin (REN) (AA 24-406) protein (GST tag)REN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Renin (REN) (Active) protein (His tag)REN Origin: Mouse Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Renin (REN) (AA 24-406) protein (His tag)REN Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
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Storage
- -20 °C,-80 °C
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Storage Comment
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- Renin (REN)
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Alternative Name
- Renin/REN
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Background
- HNFJ2,REN,renin,Renin
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Gene ID
- 5972
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UniProt
- P00797
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Pathways
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
Target
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