Renin Protein (REN) (His tag)

Catalog No. ABIN7320334

Recombinant Renin protein expressed in HEK-293 Cells.

Quick Overview for Renin Protein (REN) (His tag) (ABIN7320334)

Target: Renin (REN)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Application: ELISA

Purity: > 97 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Renin protein is labelled with His tag.

Purpose: Recombinant Mouse REN1/Renin-1 Protein (His Tag)

Sequence: Met 1-Arg 402

Characteristics: A DNA sequence encoding the pro form of mouse REN1 (NP_112469.1) (Met 1-Arg 402) was fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave the fluorogenic peptide substrate 5-FAM/QXL™ 520 (Peti-Peterdi, J. et al., 2009, Physiology 24:88.). The specific activity is > 20 pmoles/min/μg. 2. Immobilized mouse REN1-His at 10ug/ml (100 μl/well) can bind biotinylated human AGT-His with a linear range of 31. 25-250 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Renin (REN)

Alternative Name: REN1/Renin-1

Background: Angiotensin-forming enzyme,Angiotensinogenase,Kidney renin,Ren,Ren-1,Ren-A,Ren1,Ren1c,Ren1d,Renin-1,Rn-1,Rnr,Renin-1, also known as Ren-1, Angiotensinogenase and Kidney renin, is a member of thepeptidase A1 family. Renin-1 is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration. androgen and thyroid hormones influence levels of Renin-1 in mouse submandibular gland (SMG) primarily by regulating the amount of Renin-1 mRNA available for translation. Renin-1 is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It is expressed at relatively low levels in mouse SMG and kidney. Ren-2 is expressed at high levels in the mouse SMG and at very low levels, if at all, in the kidney. Ren-1 and Ren-2 are closely linked on mouse chromosome 1, show extensive homology in coding and noncoding regions and provide a model for studying the regulation of gene expression.

Molecular Weight:

Calculated MW: 43.2 kDa

Observed MW: 48-55 kDa

Gene ID: 19701

NCBI Accession: NP_112469

UniProt: P06281

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour