UBE2D3 Protein (His tag)
Quick Overview for UBE2D3 Protein (His tag) (ABIN7533726)
Target
See all UBE2D3 ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This UBE2D3 protein is labelled with His tag.
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Purpose
- Recombinant Human UBE2D3 Protein
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Sequence
- MALKRINKEL SDLARDPPAQ CSAGPVGDDM FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD RDKYNRISRE WTQKYAM
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Specificity
- Met1-Met147
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- < 0.1 EU/μg of the protein by LAL method.
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Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (AA 1-147) protein (His tag)
custom-made UBE2D3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (AA 1-147) protein (GST tag)UBE2D3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (AA 1-149) protein (His tag)UBE2D3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90% by SDS-PAGE SDS
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (AA 1-147), (full length) protein (His-SUMO Tag)UBE2D3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (Transcript Variant 9) protein (Myc-DYKDDDDK Tag)UBE2D3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)UBE2D3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
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Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
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Storage
- -20 °C,-80 °C
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Storage Comment
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Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
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- UBE2D3 (Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3))
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Alternative Name
- UBE2D3
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Background
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Description: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase.
Name: UBE2D3,E2(17)KB3,UBC4/5,UBCH5C
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Gene ID
- 7323
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UniProt
- P61077
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Pathways
- Activation of Innate immune Response, Toll-Like Receptors Cascades
Target
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