PLAP Protein (AA 21-504) (His tag)

Catalog No. ABIN7852644

Recombinant PLAP protein expressed in Mammalian Cells.

Quick Overview for PLAP Protein (AA 21-504) (His tag) (ABIN7852644)

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Protein Type: Recombinant

Origin: Cynomolgus

Source: Mammalian Cells

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 21-504

Purification tag / Conjugate: This PLAP protein is labelled with His tag.

Purpose: Recombinant Cynomolgus ALPP protein with C-terminal 10xHis tag

Specificity: ALPP (Ile21-Asp504) 10xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Alternative Name: ALPP

Background: The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. One of the main sources of this enzyme is the liver, and thus, it's one of several indicators of liver injury in different clinical conditions. In pregnant women, this protein is primarily expressed in placental and endometrial tissue, however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Aug 2020]

Molecular Weight: predicted molecular mass of 54.1 kDa after removal of the signal peptide. The apparent molecular mass of cALPP-His is 55-70 kDa due to glycosylation.

NCBI Accession: XP_045223825