VEGF 165 protein (Biotin)

Catalog No. ABIN7539353

This Recombinant protein is expressed in Escherichia coli (E. coli).

Quick Overview for VEGF 165 protein (Biotin) (ABIN7539353)

Target: VEGF 165

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE and Coomassie stain

Product Details

Purification tag / Conjugate: Biotin

Purpose: VEGF165-Biotin

Sequence: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Specificity: Chromosomal location:6p12

Characteristics: Length (aa):165

Endotoxin Level: < 0.1 ng per μg of human VEGF165

Application Details

Application Notes: Tested in a functional ELISA.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The lyophilized VEGF165-Biotin should be reconstituted in 50 mM acetic acid to a concentration not lower than 50 μg/mL. For long term storage we recommend to add at least 0.1 % human or bovine serum albumin.

Buffer: 50 mM acetic acid

Storage: -20 °C,-80 °C

Storage Comment: Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted VEGF165-Biotin should be stored in working aliquots at -20°C.

Expiry Date: 6 months

Target Details

Target: VEGF 165

Alternative Name: VEGF165

Background: Vascular endothelial growth factor A, VEGFA, VPF, VEGF, MVCD1,Human Vascular Endothelial Growth Factor VEGF165, a 23 kDa protein consisting of 165 amino acid residues, is produced as a homodimer. VEGF is a polypeptide growth factor and a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Two high-affinity tyrosine kinase receptors for VEGF165 have been identified, VEGFR-1 (FLT-1), and VEGFR-2 (KDR). Consistent with the endothelial cell-specific action of VEGF165, expression of both receptor genes has been found predominantly but not exclusively on endothelial cells. Expression of VEGFR-1 was also found on human monocytes, neutrophils (PMNs), bovine brain pericytes and villous and extra villous trophoblast. In addition to its action as a mitogen it is a potent vascular permeability factor (VPF) in vivo. VEGF165 is also a chemo attractant molecule for monocytes and endothelial cells. 5 different proteins are generated by differential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abundant form is VEGF165. Whereas VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF165 is apparently a homo-dimer, but preparations of VEGF165 show some heterogeneity on SDS gels, depending on the secretion of different glycosylation patterns. All dimeric forms have similar biological activities but their bioavailability is very different. There is good evidence that different cells and tissues express different VEGF isoforms. The other members of this increasing growth factor family are VEGF-B, -C, -D and -E. Another member is the Placenta growth factor PlGF.

Molecular Weight: 38.2 kDa

Gene ID: 7422

NCBI Accession: NM_001025366, NP_001020537

UniProt: P15692