DEPDC1 Protein (AA 1-811) (His tag)

Catalog No. ABIN7553642

This Recombinant DEPDC1 protein is expressed in HEK-293 Cells.

Quick Overview for DEPDC1 Protein (AA 1-811) (His tag) (ABIN7553642)

Target: DEPDC1 (DEP Domain Containing 1 (DEPDC1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-811

Purification tag / Conjugate: This DEPDC1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant DEPDC1 Protein expressed in mammalian cells.

Sequence: MESQGVPPGP YRATKLWNEV TTSFRAGMPL RKHRQHFKKY GNCFTAGEAV DWLYDLLRNN SNFGPEVTRQ QTIQLLRKFL KNHVIEDIKG RWGSENVDDN NQLFRFPATS PLKTLPRRYP ELRKNNIENF SKDKDSIFKL RNLSRRTPKR HGLHLSQENG EKIKHEIINE DQENAIDNRE LSQEDVEEVW RYVILIYLQT ILGVPSLEEV INPKQVIPQY IMYNMANTSK RGVVILQNKS DDLPHWVLSA MKCLANWPRS NDMNNPTYVG FERDVFRTIA DYFLDLPEPL LTFEYYELFV NILVVCGYIT VSDRSSGIHK IQDDPQSSKF LHLNNLNSFK STECLLLSLL HREKNKEESD STERLQISNP GFQERCAKKM QLVNLRNRRV SANDIMGGSC HNLIGLSNMH DLSSNSKPRC CSLEGIVDVP GNSSKEASSV FHQSFPNIEG QNNKLFLESK PKQEFLLNLH SEENIQKPFS AGFKRTSTLT VQDQEELCNG KCKSKQLCRS QSLLLRSSTR RNSYINTPVA EIIMKPNVGQ GSTSVQTAME SELGESSATI NKRLCKSTIE LSENSLLPAS SMLTGTQSLL QPHLERVAID ALQLCCLLLP PPNRRKLQLL MRMISRMSQN VDMPKLHDAM GTRSLMIHTF SRCVLCCAEE VDLDELLAGR LVSFLMDHHQ EILQVPSYLQ TAVEKHLDYL KKGHIENPGD GLFAPLPTYS YCKQISAQEF DEQKVSTSQA AIAELLENII KNRSLPLKEK RKKLKQFQKE YPLIYQKRFP TTESEAALFG DKPTIKQPML ILRKPKFRSL R Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DEPDC1 (DEP Domain Containing 1 (DEPDC1))

Alternative Name: DEPDC1

Background: DEP domain-containing protein 1A,FUNCTION: May be involved in transcriptional regulation as a transcriptional corepressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells. {ECO:0000269|PubMed:20587513}.

Molecular Weight: 93.0 kDa

UniProt: Q5TB30

AlphaFold: Q5TB30