Lipin 1 Protein (LPIN1) (AA 1-890) (His tag)

Catalog No. ABIN7554440

Product Details

Target: Lipin 1 (LPIN1)

Protein Type: Recombinant

Protein Characteristics: AA 1-890

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Lipin 1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant LPIN1 Protein expressed in mammalian cells.

Sequence: MNYVGQLAGQ VFVTVKELYK GLNPATLSGC IDIIVIRQPN GNLQCSPFHV RFGKMGVLRS REKVVDIEIN GESVDLHMKL GDNGEAFFVQ ETDNDQEVIP MHLATSPILS EGASRMECQL KRGSVDRMRG LDPSTPAQVI APSETPSSSS VVKKRRKRRR KSQLDSLKRD DNMNTSEDED MFPIEMSSDE AMELLESSRT LPNDIPPFQD DIPEENLSLA VIYPQSASYP NSDREWSPTP SPSGSRPSTP KSDSELVSKS TERTGQKNPE MLWLWGELPQ AAKSSSPHKM KESSPLSSRK ICDKSHFQAI HSESSDTFSD QSPTLVGGAL LDQNKPQTEM QFVNEEDLET LGAAAPLLPM IEELKPPSAS VVQTANKTDS PSRKRDKRSR HLGADGVYLD DLTDMDPEVA ALYFPKNGDP SGLAKHASDN GARSANQSPQ SVGSSGVDSG VESTSDGLRD LPSIAISLCG GLSDHREITK DAFLEQAVSY QQFVDNPAII DDPNLVVKIG SKYYNWTTAA PLLLAMQAFQ KPLPKATVES IMRDKMPKKG GRWWFSWRGR NTTIKEESKP EQCLAGKAHS TGEQPPQLSL ATRVKHESSS SDEERAAAKP SNAGHLPLLP NVSYKKTLRL TSEQLKSLKL KNGPNDVVFS VTTQYQGTCR CEGTIYLWNW DDKVIISDID GTITRSDTLG HILPTLGKDW THQGIAKLYH KVSQNGYKFL YCSARAIGMA DMTRGYLHWV NERGTVLPQG PLLLSPSSLF SALHREVIEK KPEKFKVQCL TDIKNLFFPN TEPFYAAFGN RPADVYSYKQ VGVSLNRIFT VNPKGELVQE HAKTNISSYV RLCEVVDHVF PLLKRSHSSD FPCSDTFSNF TFWREPLPPF ENQDIHSASA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Lipin 1 (LPIN1)

Alternative Name: LPIN1 (LPIN1 Products)

Background: Phosphatidate phosphatase LPIN1 (EC 3.1.3.4) (Lipin-1),FUNCTION: Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels (PubMed:20231281, PubMed:29765047). Is involved in adipocyte differentiation (By similarity). Acts also as nuclear transcriptional coactivator for PPARGC1A/PPARA regulatory pathway to modulate lipid metabolism gene expression (By similarity). Recruited at the mitochondrion outer membrane and is involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol (By similarity). {ECO:0000250|UniProtKB:Q91ZP3, ECO:0000269|PubMed:20231281, ECO:0000269|PubMed:29765047}.

Molecular Weight: 98.7 kDa

UniProt: Q14693

AlphaFold: Q14693

Pathways: Monocarboxylic Acid Catabolic Process