WDR6 Protein (AA 1-1121) (His tag)

Catalog No. ABIN7556001

Recombinant WDR6 protein expressed in HEK-293 Cells.

Quick Overview for WDR6 Protein (AA 1-1121) (His tag) (ABIN7556001)

Target: WDR6 (WD Repeat Domain 6 (WDR6))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1121

Purification tag / Conjugate: This WDR6 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant WDR6 Protein expressed in mammalian cells.

Sequence: MDALEDYVWP RATSELILLP VTGLECVGDR LLAGEGPDVL VYSLDFGGHL RMIKRVQNLL GHYLIHGFRV RPEPNGDLDL EAMVAVFGSK GLRVVKISWG QGHFWELWRS GLWNMSDWIW DARWLEGNIA LALGHNSVVL YDPVVGCILQ EVPCTDRCTL SSACLIGDAW KELTIVAGAV SNQLLVWYPA TALADNKPVA PDRRISGHVG IIFSMSYLES KGLLATASED RSVRIWKVGD LRVPGGRVQN IGHCFGHSAR VWQVKLLENY LISAGEDCVC LVWSHEGEIL QAFRGHQGRG IRAIAAHERQ AWVITGGDDS GIRLWHLVGR GYRGLGVSAL CFKSRSRPGT LKAVTLAGSW RLLAVTDTGA LYLYDVEVKC WEQLLEDKHF QSYCLLEAAP GPEGFGLCAM ANGEGRVKVV PINTPTAAVD QTLFPGKVHS LSWALRGYEE LLLLASGPGG VVACLEISAA PSGKAIFVKE RCRYLLPPSK QRWHTCSAFL PPGDFLVCGD RRGSVLLFPS RPGLLKDPGV GGKARAGAGA PVVGSGSSGG GNAFTGLGPV STLPSLHGKQ GVTSVTCHGG YVYTTGRDGA YYQLFVRDGQ LQPVLRQKSC RGMNWLAGLR IVPDGSMVIL GFHANEFVVW NPRSHEKLHI VNCGGGHRSW AFSDTEAAMA FAYLKDGDVM LYRALGGCTR PHVILREGLH GREITCVKRV GTITLGPEYG VPSFMQPDDL EPGSEGPDLT DIVITCSEDT TVCVLALPTT TGSAHALTAV CNHISSVRAV AVWGIGTPGG PQDPQPGLTA HVVSAGGRAE MHCFSIMVTP DPSTPSRLAC HVMHLSSHRL DEYWDRQRNR HRMVKVDPET RYMSLAVCEL DQPGLGPLVA AACSDGAVRL FLLQDSGRIL QLLAETFHHK RCVLKVHSFT HEAPNQRRRL LLCSAATDGS LAFWDLTTML DHDSTVLEPP VDPGLPYRLG TPSLTLQAHS CGINSLHTLP TREGHHLVAS GSEDGSLHVF VLAVEMLQLE EAVGEAGLVP QLRVLEEYSV PCAHAAHVTG LKILSPSIMV SASIDQRLTF WRLGHGEPTF MNSTVFHVPD VADMDCWPVS PEFGHRCALG GQGLEVYNWY D Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: WDR6 (WD Repeat Domain 6 (WDR6))

Alternative Name: WDR6

Background: TRNA (34-2'-O)-methyltransferase regulator WDR6 (WD repeat-containing protein 6),FUNCTION: Together with methyltransferase FTSJ1, methylates the 2'-O-ribose of nucleotides at position 34 of the tRNA anticodon loop of substrate tRNAs (PubMed:32558197, PubMed:33771871). Required for the correct positioning of the substrate tRNA for methylation (PubMed:32558197). Required to suppress amino acid starvation-induced autophagy (PubMed:22354037). Enhances the STK11/LKB1-induced cell growth suppression activity (PubMed:17216128). {ECO:0000269|PubMed:17216128, ECO:0000269|PubMed:22354037, ECO:0000269|PubMed:32558197, ECO:0000269|PubMed:33771871}.

Molecular Weight: 121.7 kDa

UniProt: Q9NNW5

AlphaFold: Q9NNW5