TAF1B Protein (AA 1-586) (His tag)

Catalog No. ABIN7556790

Recombinant TAF1B protein expressed in HEK-293 Cells.

Quick Overview for TAF1B Protein (AA 1-586) (His tag) (ABIN7556790)

Target: TAF1B (TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase I, B, 63kDa (TAF1B))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-586

Purification tag / Conjugate: This TAF1B protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Taf1b Protein expressed in mammalian cells.

Sequence: MDVEEVKAFR DRCSQCAAVS WGLTDEGKYY CTSCHNVTDR SEEVVSAADI PNTKINSINR GLRQRSKHEK GWDWYVCEGF QCILYHQAKA LETLGVSPEL KNEVLHNFWK RYLQKSKQAY CKNPVRTSGR KAKVLEDSVQ SSDLGSDLEL LSDTTCPLES EAEFQSDPQI PKPFPVTKGS PKSASVCSGS VDGVEYSERK EKGLVKMTVP RTLALCSLSL LWQRETITVS DLLRFVEEDH IPYINAFKLF PEEMKVYGRD KGIFAIESWP DYEDIYKNMI ELAIFLDLPR FPDITEDCYL HPNTLCMKYL LEVNLPDEMH TLTCQVVKLT GIGEVDFLTF DPIAKTKRTV KYDVQAMAVI VVVLKLLFLL DDKLEWSYSD LAEAYNEQHR EDTPQFDFRK WYQVMKKTFD EKRRKWEEAR ARYAWKTKRP LYRSHIDKSV AYKRRKMVEN LQKQFSALVG SSPVVEKQAP SSFQFNWTEE GTDSPCFHGH SLQGLLIMKG QSMITKNSLY WLSTQKFCKS YCKHVTTYEE SNFSLSYQFI LNIFSFLLRI KTSALHEEVS LLEKKLFEKK YNESKKSSGS KKGRRH Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TAF1B (TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase I, B, 63kDa (TAF1B))

Alternative Name: Taf1b

Background: TATA box-binding protein-associated factor RNA polymerase I subunit B (RNA polymerase I-specific TBP-associated factor 68 kDa) (TAFI68) (TATA box-binding protein-associated factor 1B) (TBP-associated factor 1B) (Transcription initiation factor SL1/TIF-IB subunit B),FUNCTION: Component of RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during transcription initiation such as pre-initiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. Binds rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex and, possibly, directly through its interaction with RRN3. {ECO:0000269|PubMed:9050847}.

Molecular Weight: 68.0 kDa

UniProt: P97358

AlphaFold: P97358