TTLL12 Protein (AA 1-639) (His tag)

Catalog No. ABIN7557169

This Recombinant TTLL12 protein is produced in HEK-293 Cells.

Quick Overview for TTLL12 Protein (AA 1-639) (His tag) (ABIN7557169)

Target: TTLL12 (Tubulin tyrosine Ligase-Like Family, Member 12 (TTLL12))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-639

Purification tag / Conjugate: This TTLL12 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Ttll12 Protein expressed in mammalian cells.

Sequence: MEIQSGPQPG SPGRAERLNA RLLDEFVSLH GPTLRASGVP ERLWGRLLHK LEHEVFDAGE MFGIMQVEEV EEAEDEAARE AQRKQPNPGG ELCYKVIVTS ESGVRADDPN SIFLIDHAWT CRVEHARKQL QQVPGLLHRM ANLMGIEFHG EVPSPEVVAL VLEEMWKFNQ TYQLAHGTAE EKVPVWYIMD EFGSRIQHSD MPSFATAPFF YMPQQVAYTL LWPLRDLDTG EEVTRDFAYG EADPLIRKCM LLPWAPADML DLSFSTPEPP AKYYQAILEE NKEKLPLAIS PVARPQGHVF RVHCDVQQVL GHLTHPRFTF TDSEADADIF FHFSHFKDYM KLSQESPQVL LNQFPCENLL TVKDCLASIA RRAGGPEGPP WLPRTFNLRT ELPQFVSYFQ HRERRGEDNH WICKPWNLAR SLDTHVTNNL HSIIRHREST PKVVSKYIES PVLFLREDVG NVKFDIRYIV LLRSVRPLRL FAYDVFWLRF SNRPFALDDL DDYEKHFTVM NYDPDVVLKQ VHYNEFIPQF EKQYPEFPWS DVQAEIFKAF TELFQVACAK PPPMGLCDYP SSRAMYAIDL MLNWDNHPDG KRVMQPQILE VNFNPDCERA CRYHPSFFND VFSTLFLDET DNCHVTRII Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TTLL12 (Tubulin tyrosine Ligase-Like Family, Member 12 (TTLL12))

Alternative Name: Ttll12

Background: Tubulin--tyrosine ligase-like protein 12 (Inactive tubulin--tyrosine ligase-like protein 12),FUNCTION: Negatively regulates post-translational modifications of tubulin, including detyrosination of the C-terminus and polyglutamylation of glutamate residues. Also, indirectly promotes histone H4 trimethylation at 'Lys-20' (H4K20me3). Probably by controlling tubulin and/or histone H4 post-translational modifications, plays a role in mitosis and in maintaining chromosome number stability. During RNA virus-mediated infection, acts as a negative regulator of the RIG-I pathway by preventing MAVS binding to TBK1 and IKBKE. {ECO:0000250|UniProtKB:Q14166}.

Molecular Weight: 74.0 kDa

UniProt: Q3UDE2

AlphaFold: Q3UDE2