JMJD4 Protein (AA 1-427) (His tag)

Catalog No. ABIN7558496

This Recombinant JMJD4 protein is expressed in HEK-293 Cells.

Quick Overview for JMJD4 Protein (AA 1-427) (His tag) (ABIN7558496)

Target: JMJD4 (Jumonji Domain Containing 4 (JMJD4))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-427

Purification tag / Conjugate: This JMJD4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Jmjd4 Protein expressed in mammalian cells.

Sequence: MDRETRTFAE RYYRDLRDPV PSGGGGPTPS GVTFIQTPNA FSYADFVKGF LLPNLPCVFS SAFTEGWGSR RRWVTSEGKP DFEYLQQKYG DAVVPVANCG VREYNSNPKE HMSFRDYISY WKDYIQGSYS SSRGCLYLKD WHLCRDSLVN DLEDIFTLPV YFSSDWLNEF WDVLNVDDYR FVYAGPRGTW SPFHADIFRS FSWSVNICGK KKWLFFPPGE EEALRDCHGN LPYDVTSTEL LDTHLYPKIQ HHSLPIEVIQ EPGEMVFVPS GWHHQVYNLD DTISINHNWV NGCNLPNMWH FLQQELQAVQ HEVEEWKDSM PDWHHHCQVI MKSCTGINFE EFYHFLKVIA EKRLLVLEQG LKGDSGDSRS LDLGLQQAAF DIGRLADVLA SVVVNPDFQR VDTSAFSPQP EELLQQLEDA VAAAEAL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: JMJD4 (Jumonji Domain Containing 4 (JMJD4))

Alternative Name: Jmjd4

Background: 2-oxoglutarate and iron-dependent oxygenase JMJD4 (EC 1.14.11.-) (JmjC domain-containing protein 4) (Jumonji domain-containing protein 4) (Lysyl-hydroxylase JMJD4),FUNCTION: Catalyzes the 2-oxoglutarate and iron-dependent C4-lysyl hydroxylation of ETF1 at 'Lys-63' thereby promoting the translational termination efficiency of ETF1 (By similarity). Not essential for embryonic stem cell (ESC) maintenance and the embryonic and postnatal development (PubMed:27147518). {ECO:0000250|UniProtKB:Q9H9V9, ECO:0000269|PubMed:27147518}.

Molecular Weight: 49.2 kDa

UniProt: Q8BFT6

AlphaFold: Q8BFT6