P4HTM Protein (AA 1-503) (His tag)

Catalog No. ABIN7558529

Product Details

Target: P4HTM (Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM))

Protein Type: Recombinant

Protein Characteristics: AA 1-503

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This P4HTM protein is labelled with His tag.

Purpose: Predefined custom protein recombinant P4htm Protein expressed in mammalian cells.

Sequence: MAAAVATVQR PEAETVEEAS NLQWPLPPEH RPSGAATRPG DSEDAPVRPL CKPRGICSRA YFLVLMVFVH LYLGNVLALL LFVHYSNGDE STDPGPQRRE QSPQPVPTLG PLTRLEGIKV GYERKVQVVA GRDHFIRTLS LKPLLFEIPG FLSDEECRLI IHLAQMKGLQ RSQILPTEEY EEAMSAMQVS QLDLFQLLDQ NHDGRLQLRE VLAQTRLGNG RWMTPENIQE MYSAIKADPD GDGVLSLQEF SNMDLRDFHK YMRSHKAESN ELVRNSHHTW LHQGEGAHHV MRAIRQRVLR LTRLSPEIVE FSEPLQVVRY GEGGHYHAHV DSGPVYPETI CSHTKLVANE SVPFETSCRY MTVLFYLNNV TGGGETVFPV ADNRTYDEMS LIQDDVDLRD TRRHCDKGNL RVKPQQGTAV FWYNYLPDGQ GWVGEVDDYS LHGGCLVTRG TKWIANNWIN VDPSRARQAL FQQEMARLAR EGGMDSQPEW ALDRAYSDAR VEL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: P4HTM (Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM))

Alternative Name: P4htm (P4HTM Products)

Background: Transmembrane prolyl 4-hydroxylase (P4H-TM) (EC 1.14.11.29) (Hypoxia-inducible factor prolyl hydroxylase 4) (HIF-PH4) (HIF-prolyl hydroxylase 4) (HPH-4),FUNCTION: Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. {ECO:0000250|UniProtKB:Q9NXG6}.

Molecular Weight: 57.0 kDa

UniProt: Q8BG58

AlphaFold: Q8BG58