P4HTM Protein (AA 1-563) (GST tag)
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- Target See all P4HTM Proteins
- P4HTM (Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-563
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This P4HTM protein is labelled with GST tag.
- Application
- ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)
- Purpose
- P4HTM (Human) Recombinant Protein (P01)
- Sequence
- MAAAAVTGQR PETAAAEEAS RPQWAPPDHC QAQAAAGLGD GEDAPVRPLC KPRGICSRAY FLVLMVFVHL YLGNVLALLL FVHYSNGDES SDPGPQHRAQ GPGPEPTLGP LTRLEGIKVG HERKVQLVTD RDHFIRTLSL KPLLFEIPGF LTDEECRLII HLAQMKGLQR SQILPTEEYE EAMSTMQVSQ LDLFRLLDQN RDGHLQLREV LAQTRLGNGW WMTPESIQEM YAAIKADPDG DGVLSLQEFS NMDLRDFHKY MRSHKAESSE LVRNSHHTWL YQGEGAHHIM RAIRQRVLRL TRLSPEIVEL SEPLQVVRYG EGGHYHAHVD SGPVYPETIC SHTKLVANES VPFETSCRQV SPNWGLPSIL RPGTPMTQAQ PCTVGVPLGM GPGDHWVIPV SPWEHPQLGT CSVPPLPYSY MTVLFYLNNV TGGGETVFPV ADNRTYDEMS LIQDDVDLRD TRRHCDKGNL RVKPQQGTAV FWYNYLPDGQ GWVGDVDDYS LHGGCLVTRG TKWIANNWIN VDPSRARQAL FQQEMARLAR EGGTDSQPEW ALDRAYRDAR VEL
- Characteristics
- Human P4HTM full-length ORF ( NP_808807.2, 1 a.a. - 563 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM) (AA 82-502) protein (His tag)
Crystallography grade P4HTM Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM) (AA 83-503) protein (His tag)Crystallography grade P4HTM Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM) (AA 1-502) protein (Strep Tag)Crystallography grade P4HTM Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM) (AA 1-503) protein (Strep Tag)Crystallography grade P4HTM Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM) (AA 82-563) protein (His-SUMO Tag)P4HTM Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- P4HTM (Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM))
- Alternative Name
- P4HTM (P4HTM Products)
- Synonyms
- Ph-4 Protein, RGD1311848 Protein, EGLN4 Protein, HIFPH4 Protein, P4H-TM Protein, PH-4 Protein, PH4 Protein, PHD4 Protein, 4933406E20Rik Protein, AI853847 Protein, BB128974 Protein, prolyl 4-hydroxylase, transmembrane Protein, prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) Protein, shisa family member 5 Protein, P4htm Protein, P4HTM Protein, SHISA5 Protein
- Background
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Full Gene Name: prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum)
Synonyms: EGLN4,HIFPH4,P4H-TM,PH-4,PH4,PHD4 - Gene ID
- 54681
- NCBI Accession
- NM_177938
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