GSKIP Protein (AA 1-139) (His tag)

Catalog No. ABIN7558577

This Recombinant GSKIP protein is expressed in HEK-293 Cells.

Quick Overview for GSKIP Protein (AA 1-139) (His tag) (ABIN7558577)

Target: GSKIP (GSK3B interacting protein (GSKIP))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-139

Purification tag / Conjugate: This GSKIP protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Gskip Protein expressed in mammalian cells.

Sequence: METDYNPVEL SSMSGFEEGS ELNGFEGADM KDMQLEAEAV VNDVLFAVNH MFVSKSMPCA DDVAYINVET KERNRYCLEL TEAGLRVVGY AFDQVEDHLQ TPYHETVYSL LDTLSPAYRE AFGNALLQRL EALKRDGQS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: GSKIP (GSK3B interacting protein (GSKIP))

Alternative Name: Gskip

Background: GSK3B-interacting protein (GSKIP),FUNCTION: A-kinase anchoring protein for GSK3B and PKA that regulates or facilitates their kinase activity towards their targets. The ternary complex enhances Wnt-induced signaling by facilitating the GSK3B- and PKA-induced phosphorylation of beta-catenin leading to beta-catenin degradation and stabilization respectively. Upon cAMP activation, the ternary complex contributes to neuroprotection against oxidative stress-induced apoptosis by facilitating the PKA-induced phosphorylation of DML1 and PKA-induced inactivation of GSK3B. During neurite outgrowth promotes neuron proliferation, while increases beta-catenin-induced transcriptional activity through GSK3B kinase activity inhibition, reduces N-cadherin level to promote cell cycle progression (By similarity). May play a role in cleft palate formation and is required for postnatal life through modulation of the activity of GSK3B during development (PubMed:26582204). {ECO:0000250|UniProtKB:Q9P0R6, ECO:0000269|PubMed:26582204}.

Molecular Weight: 15.6 kDa

UniProt: Q8BGR8

AlphaFold: Q8BGR8