PTGES2 Protein (AA 1-384) (His tag)

Catalog No. ABIN7558965

Recombinant PTGES2 protein expressed in HEK-293 Cells.

Quick Overview for PTGES2 Protein (AA 1-384) (His tag) (ABIN7558965)

Target: PTGES2 (Prostaglandin E Synthase 2 (PTGES2))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-384

Purification tag / Conjugate: This PTGES2 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Ptges2 Protein expressed in mammalian cells.

Sequence: MAQAARLSWV LVSSRCALTE GLLTRPWQPL SAQSRAGFTR VAAGSRGAAV RKGSPRLLGA AALALGGALG LYHTVRWHQR SQDLRAERSA AQLPLSNSLQ LTLYQYKTCP FCSKVRAFLD FHSLPYQVVE VNPVRRTEIK FSSYRKVPIL VAQEGDSLQQ LNDSSVIISA LKTYLVSGQP LEEVITYYPP MKAMNDQGKE VTEFCNKYWL MLDEKEAQQM YGGKEARTEE MKWRQWADDW LVHLISPNVY RTPAEALASF DYIVREGKFG AVEAAMAKYV GAAAMYLISK RLKSRHHLQD DVRVDLYEAA NKWVTAVGKD RPFMGGQKPN LADLAVYGVL RVMEGLEAFD DLMRHSHIQP WYLRMERAIE EAPSVHHVNP SCKD Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PTGES2 (Prostaglandin E Synthase 2 (PTGES2))

Alternative Name: Ptges2

Background: Prostaglandin E synthase 2 (EC 5.3.99.3) (GATE-binding factor 1) (GBF-1) (Microsomal prostaglandin E synthase 2) (mPGES-2) [Cleaved into: Prostaglandin E synthase 2 truncated form],FUNCTION: Isomerase that catalyzes the conversion of PGH2 into the more stable prostaglandin E2 (PGE2) (in vitro). The biological function and the GSH-dependent property of PTGES2 is still under debate (By similarity). In vivo, PTGES2 could form a complex with GSH and heme and would not participate in PGE2 synthesis but would catalyze the degradation of prostaglandin E2 H2 (PGH2) to 12(S)-hydroxy-5(Z),8(E),10(E)-heptadecatrienoic acid (HHT) and malondialdehyde (MDA) (By similarity). May also have transactivation activity toward IFN-gamma (IFNG), possibly via an interaction with CEBPB, however, the relevance of transcription activation activity remains unclear (PubMed:12050152). {ECO:0000250|UniProtKB:Q9H7Z7, ECO:0000250|UniProtKB:Q9N0A4, ECO:0000303|PubMed:12050152}.

Molecular Weight: 43.3 kDa

UniProt: Q8BWM0

AlphaFold: Q8BWM0

Pathways: Interferon-gamma Pathway, Cell RedoxHomeostasis, SARS-CoV-2 Protein Interactome