SLX1A/GIYD2 Protein (AA 1-270) (His tag)

Catalog No. ABIN7558985

This Recombinant SLX1A/GIYD2 protein is expressed in HEK-293 Cells.

Quick Overview for SLX1A/GIYD2 Protein (AA 1-270) (His tag) (ABIN7558985)

Target: SLX1A/GIYD2 (SLX1B) (SLX1 Structure-Specific Endonuclease Subunit Homolog B (SLX1B))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-270

Purification tag / Conjugate: This SLX1A/GIYD2 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Slx1b Protein expressed in mammalian cells.

Sequence: MDHAARPGRF FGVYLLYCQN PRHRGRVYVG FTVNPARRVR QHNAGRKKGG AWRTSGRGPW DMVLIIHGFP SAVAALRFEW AWQHPQASRR LTHVGPRLRS EAAFAFHLRV LAHMLRVPPW VRLPLTLRWL RPDFRHELCP APPAHMPIAF GPPPPQPLVP KRPAVSEADS ERQLDLGTKA RCSLCARLLQ DEEGPLCCPH PGCPLRAHII CLAEEFLQEE PGQLLPLEGH CPSCKKSLLW GNLVGQCHAD TEEEEDLELE EEHWTDLLET Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SLX1A/GIYD2 (SLX1B) (SLX1 Structure-Specific Endonuclease Subunit Homolog B (SLX1B))

Alternative Name: Slx1b

Background: Structure-specific endonuclease subunit SLX1 (EC 3.1.-.-) (GIY-YIG domain-containing protein 1),FUNCTION: Catalytic subunit of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products. {ECO:0000255|HAMAP-Rule:MF_03100}.

Molecular Weight: 30.7 kDa

UniProt: Q8BX32

AlphaFold: Q8BX32

Pathways: DNA Damage Repair