ERCC8 Protein (AA 1-397) (His tag)

Catalog No. ABIN7559303

This Recombinant ERCC8 protein is expressed in HEK-293 Cells.

Quick Overview for ERCC8 Protein (AA 1-397) (His tag) (ABIN7559303)

Target: ERCC8 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 (ERCC8))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-397

Purification tag / Conjugate: This ERCC8 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Ercc8 Protein expressed in mammalian cells.

Sequence: MLSFLSARQS GLEDPLRLRR AQSTRRVLGL ELNKDRDVER IHGSGVNTLD IEPVEGRYML SGGSDGVVVL YDLENASRQP HYTCKAVCSV GRSHPDVHKY SVETVQWYPH DTGMFTSSSF DKTLKVWDTN TLQAADVFNF EETVYSHHMS PAATKHCLVA VGTRGPKVQL CDLKSGSCSH ILQGHRQEIL AVSWSPRHDY ILATASADSR VKLWDVRRAS GCLLTLDQHN GKKSQAAESA NTAHNGKVNG LCFTSDGLHL LTIGTDNRMR LWNSSSGDNT LVNYGKVCND SRKGLQFAVS CGCSSEFVFV PHGSTIAVYA VHSGERLAML KGHYKSVDCC VFQPNFQELY SGSRDCNILA WVPPSYEPVP DDDDEAPAKS QLNPAFADAW SSSDEDG Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ERCC8 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 (ERCC8))

Alternative Name: Ercc8

Background: DNA excision repair protein ERCC-8 (Cockayne syndrome WD repeat protein CSA homolog),FUNCTION: Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair (By similarity). The CSA complex (DCX(ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner, ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair (By similarity). It is required for the recruitment of XAB2, HMGN1 and TCEA1/TFIIS to a transcription-coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes (By similarity). Plays a role in DNA single-strand and double-strand breaks (DSSBs) repair, involved in repair of DSSBs by non-homologous end joining (NHEJ) (By similarity). {ECO:0000250|UniProtKB:Q13216}.

Molecular Weight: 43.7 kDa

UniProt: Q8CFD5

AlphaFold: Q8CFD5

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus