TRIM29 Protein (AA 1-587) (His tag)

Catalog No. ABIN7559699

This Recombinant TRIM29 protein is expressed in HEK-293 Cells.

Quick Overview for TRIM29 Protein (AA 1-587) (His tag) (ABIN7559699)

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-587

Purification tag / Conjugate: This TRIM29 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Trim29 Protein expressed in mammalian cells.

Sequence: MEGADACRSN GASPEARDTR SPPGPSGSLE NGTKADSKDT KTTNGHSGEV TEGKTLGSAL KSGEGKSGLF SSNEWRRPII QFVESVDDKG SSYFSMDSAE GRRSPYAGLQ LGASKKPPVT FAEKGELRKS IFSEPRKPTV TIVEPGEVRR NSYPRADSSL LARAKSGSEE VLCDSCIGNK QKAVKSCLVC QASFCELHLK PHLEGAAFRD HQLLEPIRDF EARKCPLHGK TMELFCQTDQ TCICYLCMFQ EHKNHSTVTV EEAKAEKETE LSLQKEQLQL KIIEIEDDVE KWQKEKDRIK SFTTNEKAIL EQNFRDLVRE LEKQKEEVRA ALEQREQDAV DQVKVIVDAL DERAKVLHED KQTREQLHNI SDSVLFLQEF GALMSNYSLP PPLPTYHVLL EGEGLGQSLG NCKDDLLNVC MRHVEKMCKA DLSRNFIERN HMENGGDHRY MNSYTSSYGN EWSTPDTMKR YSMYLTPKGG GRTSYQPSSP SRLSKETNQK NFNNLYGTKG NYTSRVWEYT STVQNSEDMP TVQGNSSFSL KGFPSLLRSQ VPKAQPQTWK SGKQTLLSHY RPFYVNKGSG IGSNEAP Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Alternative Name: Trim29

Background: Tripartite motif-containing protein 29,FUNCTION: Plays a crucial role in the regulation of macrophage activation in response to viral or bacterial infections within the respiratory tract. Mechanistically, TRIM29 interacts with IKBKG/NEMO in the lysosome where it induces its 'Lys-48' ubiquitination and subsequent degradation. In turn, the expression of type I interferons and the production of pro-inflammatory cytokines are inhibited. Additionally, induces the 'Lys-48' ubiquitination of STING1 in a similar way, leading to its degradation. {ECO:0000250|UniProtKB:Q14134}.

Molecular Weight: 65.8 kDa

UniProt: Q8R2Q0

AlphaFold: Q8R2Q0

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus