CYP2C70 Protein (AA 1-489) (His tag)

Catalog No. ABIN7560016

This Recombinant CYP2C70 protein is produced in HEK-293 Cells.

Quick Overview for CYP2C70 Protein (AA 1-489) (His tag) (ABIN7560016)

Target: CYP2C70 (Cytochrome P450, Family 2, Subfamily C, Polypeptide 70 (CYP2C70))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-489

Purification tag / Conjugate: This CYP2C70 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Cyp2c70 Protein expressed in mammalian cells.

Sequence: MALFIFLGIW LSCFLFLFLW NQHRGRGKLP PGPTPLPIVG NILQVDVKNI SKSMGMLAKK YGPVFTVYLG MKPTVVLHGY KAMKEALIDQ GDEFSDKTDS SLLSRTSQGL GIVFSNGETW KQTRRFSLMV LRSMGMGKKT IEDRIQEEIL YMLDALRKTN GSPCDPSFLL ACVPCNVIST VIFQHRFDYN DQTFQDFMEN FHRKIEILAS PWSQLCSAYP ILYYLPGIHN RFLKDVTQQK KFILEEINRH QKSLDLSNPQ DFIDYFLIKM EKEKHNQKSE FTMDNLVVSI GDLFGAGTET TSSTVKYGLL LLLKYPEVTA KIQEEIAHVI GRHRRPTMQD RNHMPYTDAV LHEIQRYIDF VPIPSPRKTT QDVEFRGYHI PKGTSVMACL TSVLNDDKEF PNPEKFDPGH FLDEKGNFKK SDYFVAFSAG RRACIGEGLA RMEMFLILTN ILQHFTLKPL VKPEDIDTKP VQTGLLHVPP PFELCFIPV Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: CYP2C70 (Cytochrome P450, Family 2, Subfamily C, Polypeptide 70 (CYP2C70))

Alternative Name: Cyp2c70

Background: Cytochrome P450 2C70 (EC 1.14.14.-) (CYPIIC70),FUNCTION: A cytochrome P450 monooxygenase involved in muricholic acid (MCA) synthesis (PubMed:27638959). Hydroxylates at the 6-beta position two major bile acids, chenodeoxycholic acid (CDCA) and ursodeoxycholic acid (UDCA) to form alpha-MCA and beta-MCA, respectively (PubMed:27638959). May regulate NR1H4/farnesoid X receptor signaling, as taurine-conjugated MCAs are antagonists of NR1H4. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR, NADPH-ferrihemoprotein reductase) (PubMed:27638959). {ECO:0000269|PubMed:27638959}.

Molecular Weight: 56.0 kDa

UniProt: Q91W64

AlphaFold: Q91W64