ATPBD3 Protein (AA 1-420) (His tag)

Catalog No. ABIN7560293

This Recombinant ATPBD3 protein is expressed in HEK-293 Cells.

Quick Overview for ATPBD3 Protein (AA 1-420) (His tag) (ABIN7560293)

Target: ATPBD3 (CTU1) (Cytosolic Thiouridylase Subunit 1 Homolog (CTU1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-420

Purification tag / Conjugate: This ATPBD3 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Ctu1 Protein expressed in mammalian cells.

Sequence: MPAPTCFSCH KTRAALRRPR SGQALCGSCF CAAFEAEVLH TVLAGHLLPP GAVVAVGASG GKDSTVLAHV LRELAPRLGI TLHLVAVDEG IGGYRDAALE AVRSQAARWE LPLTIVAYED LFGGWTMDAV ARSTAGSGRS RSCCTFCGVL RRRALEEGAR LVGATHIVTG HNADDMAETV LMNFLRGDAG RLARGGVLGS TGEGCALPRC RPLQFASQKE VVLYAHFRHL RYFSEECVYA PEAFRGHARD LLKLLEAARP SAVLDLVHSA ERLALAPAAK PPPPGTCSRC GALASHKLCQ ACALLDGLNR GLPRLAIGKG RRVLQVEPPQ PGNPSLVTSD PVAPAGPCTC KQPKDKANPC GNGGDRAGAT CVSQCDLSPG NGEDRAGATC VSQRDLSLGN GGDRAGATCV SQCDLSPVAE Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ATPBD3 (CTU1) (Cytosolic Thiouridylase Subunit 1 Homolog (CTU1))

Alternative Name: Ctu1

Background: Cytoplasmic tRNA 2-thiolation protein 1 (EC 2.7.7.-) (ATP-binding domain-containing protein 3) (Cytoplasmic tRNA adenylyltransferase 1),FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. {ECO:0000255|HAMAP-Rule:MF_03053}.

Molecular Weight: 43.8 kDa

UniProt: Q99J10

AlphaFold: Q99J10