SPEF1 Protein (AA 1-234) (His tag)

Catalog No. ABIN7560314

This Recombinant SPEF1 protein is expressed in HEK-293 Cells.

Quick Overview for SPEF1 Protein (AA 1-234) (His tag) (ABIN7560314)

Target: SPEF1 (Sperm Flagellar 1 (SPEF1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-234

Purification tag / Conjugate: This SPEF1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Spef1 Protein expressed in mammalian cells.

Sequence: MESSVDEEAL HQLYLWVDNI PLSRPKRNLS RDFSDGVLVA ELIKFYFPKM VEMHNYVPAN SLQQKLSNWG HLNRKVLNKL NFSVPDDVMR KIAQCSPGVV ELVLIPLRQR LEERQRRQKL GVGSLQELAP QDSSGYMDMG LPQKVRGEGA PALGEQLREG RPLASRPPGY NQALQGDPSF VLQIAEKEQE LLASQETVQV LQMKVKRLEH LLQLKNVRID DLSRRLQQAE RKQR Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SPEF1 (Sperm Flagellar 1 (SPEF1))

Alternative Name: Spef1

Background: Sperm flagellar protein 1 (Calponin-homology and microtubule-associated protein),FUNCTION: Microtubule-associated protein that promotes microtubule bundling and stabilizes microtubules against depolymerization in response to cold shock (PubMed:16206169). Microtubule-associated protein involved in the stabilization of microtubules along the axis of migration during radial intercalation. Promotes the establishment and stabilization of an axis of microtubules required for the active migration of cells into the outer epithelium (By similarity). Essential for ciliary central apparatus formation which requires both its microtubule-binding and bundling activities and for ciliary localization of HYDIN and SPAG6 in ependymal cilia (PubMed:30535028). Binds actin in intestinal epithelial cells (IECs), essential for IECs survival and contributes to formation of filopodia and lamellipodia in migrating IECs (By similarity). Regulates planar cell polarity signaling pathway and asymmetric microtubule accumulation in ciliated epithelia (By similarity). {ECO:0000250|UniProtKB:Q0IH24, ECO:0000269|PubMed:16206169, ECO:0000269|PubMed:30535028}.

Molecular Weight: 26.8 kDa

UniProt: Q99JL1

AlphaFold: Q99JL1