TRIM34A Protein (AA 1-485) (His tag)

Catalog No. ABIN7560466

This Recombinant TRIM34A protein is produced in HEK-293 Cells.

Quick Overview for TRIM34A Protein (AA 1-485) (His tag) (ABIN7560466)

Target: TRIM34A (Tripartite Motif-Containing 34A (TRIM34A))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-485

Purification tag / Conjugate: This TRIM34A protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Trim34a Protein expressed in mammalian cells.

Sequence: MASTGLTNIQ EKTTCPVCQE LLTKALSLGC GHRVCQACLI TKKNAVINPR EKSSCPVCGT RFSLENLQAN KHLANVVERL GEVKLKPDIG TKRDLCVHHG EKLLLFCKED KKAICWVCER SQEHRGHHTF LWEEAVRECQ ENLQKALTRL RKEQEKVETL EADIKEDRLS WKCQVQTERQ RIQTGFNQLR RILDKEEQRE LKRLREEEQM ILDSLAGAEA ELAQQSQLVE ELISDLELRR EWSDTELLQD MSGILKWSQI WTLKKPKAVS KKLSMVFQAP DLSGMLQKFR ELTAVRAYWD NFTFNPENLN LNLILSEDHR QVTSVSIWPF KCCNNGILGS KCFSSGKHYW EVDVSEKKAW TLGVYTRKRT LRFDVRQRKG QPNGYHRYKP QNGYWVIGLQ HGSKYSIFED SSNCDPTVLN PFVATPLHRV GVFLDCEEGT VSFLNVTNHG SLIYKFSQCC FSQPAYPYFN PWDCPAPMTL CPLNS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TRIM34A (Tripartite Motif-Containing 34A (TRIM34A))

Alternative Name: Trim34a

Background: E3 ubiquitin-protein ligase TRIM34A (EC 2.3.2.27) (Tripartite motif-containing protein 34A),FUNCTION: Functions as antiviral protein and contributes to the defense against retroviral infections (By similarity). Acts as a capsid-specific restriction factor with the help of TRIM5 and prevents infection from non-host-adapted retroviruses. During influenza A virus infection, promotes programmed cell death by targeting ZBP1 for 'Lys-63'-linked polyubiquitination. In turn, promotes ZBP1 recruitment of RIPK3 to mediate virus-induced programmed necrosis (By similarity). Negatively regulates the function of mitochondria by enhancing mitochondrial depolarization leading to cytochrome c release and mitochondria-dependent apoptosis. Promotes also the formation of multinucleated giant cells by means of cell fusion and phagocytosis in epithelial cells (By similarity). Plays an essential role in sustaining the integrity of the inner mucus layer in the colon by controlling the exocytosis of the major component of colonic mucus MUC2 from colonic goblet cells (PubMed:32094504). {ECO:0000250|UniProtKB:Q9BYJ4, ECO:0000269|PubMed:32094504}.

Molecular Weight: 56.0 kDa

UniProt: Q99PP6

AlphaFold: Q99PP6