PSMA8 Protein (AA 1-250) (His tag)

Catalog No. ABIN7560665

This Recombinant PSMA8 protein is expressed in HEK-293 Cells.

Quick Overview for PSMA8 Protein (AA 1-250) (His tag) (ABIN7560665)

Target: PSMA8 (Proteasome Subunit alpha 8 (PSMA8))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-250

Purification tag / Conjugate: This PSMA8 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Psma8 Protein expressed in mammalian cells.

Sequence: MASRYDRAIT VFSPDGHLFQ VEYAQEAVKK GSTAVGIRGT NIVVLGVEKK SVAKLQDERT VRKICALDDH VCMAFAGLTA DARVVISRAR VECQSHKLTV EDPVTVEYIT RFIATLKQKY TQSNGRRPFG ISALIVGFDD DGIPRLYQTD PSGTYHAWKA NAIGRSAKTV REFLEKNYTE DAISNDKEAI KLAIKALLEV VQSGGKNIEL AIIRRDQPLK MFSAKEIELE VSEIEREKDE AEKTKSKKST Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PSMA8 (Proteasome Subunit alpha 8 (PSMA8))

Alternative Name: Psma8

Background: Proteasome subunit alpha type-8 (Proteasome alpha 4 subunit) (Alpha4s) (Proteasome subunit alpha-type 7-like),FUNCTION: Component of the spermatoproteasome, a proteasome specifically found in testis that promotes acetylation-dependent degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis (PubMed:23706739, PubMed:31358751, PubMed:31437213). The proteasome is a protein complex that degrades unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds (Probable). Required for 20S core proteasome assembly, essential for the degradation of meiotic proteins RAD51 and RPA1 at late prophase I and the progression of meiosis I during spermatogenesis (PubMed:31358751). Localizes to the synaptonemal complex, a 'zipper'-like structure that holds homologous chromosome pairs in synapsis during meiotic prophase I (PubMed:31437213). {ECO:0000269|PubMed:23706739, ECO:0000269|PubMed:31358751, ECO:0000269|PubMed:31437213, ECO:0000305}.

Molecular Weight: 27.9 kDa

UniProt: Q9CWH6

AlphaFold: Q9CWH6

Pathways: DNA Replication, Synthesis of DNA