PUS10 Protein (AA 1-527) (His tag)

Catalog No. ABIN7560959

This Recombinant PUS10 protein is expressed in HEK-293 Cells.

Quick Overview for PUS10 Protein (AA 1-527) (His tag) (ABIN7560959)

Target: PUS10 (Pseudouridylate Synthase 10 (PUS10))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-527

Purification tag / Conjugate: This PUS10 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Pus10 Protein expressed in mammalian cells.

Sequence: MLPLTEENKH VAQLLFSSGT CPRCILRFCG VDLPAPYKHP SKELLNELQK FLEPEKPELI LEAPNPPLKK IRLHEDGIDN LSEDGKEGVS VTEDESMAEK PSKLRVCNVC LGILQEFCEK GFITKVCQKV EASGFEFTSV VLSVSFPPQL SVREHAAWLL VKQEMGKQSL SLGRNDVVQL KEAYKWITHP LFSEELGVPT DGKSLFEVSV VFAHPETAED CHFLGEVCRD CFKPAKNKQS VFTRMAVLKA LSKIKEEDFL GQFPCPPNSP KTVCTVLEVE CTHGAVFVAG RYNKYSRNLP QTPWIIDGER KMESSVEELI SDHLLAVFRA ESFNFSSSGR EDVDVRTLGN GRPFAVELLN PHRVHFTSQE MKELQQTINK SSDKIQVRDL QLVTREAIGH MKEGEEEKTK TYSALIWTNR AIQKKDIGFL DDLKDLKIDQ KTPLRVLHRR PLAVRTRAIH SMKTHYLDEH HFRLHLKTQA GTYIKEFVHG DFGRTKPNLG SLMNVTADIL ELDVESVDVD WPPALDD Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PUS10 (Pseudouridylate Synthase 10 (PUS10))

Alternative Name: Pus10

Background: TRNA pseudouridine synthase Pus10 (EC 5.4.99.25) (Coiled-coil domain-containing protein 139) (tRNA pseudouridine 55 synthase) (Psi55 synthase) (tRNA pseudouridylate synthase) (tRNA-uridine isomerase),FUNCTION: Protein with different functions depending on its subcellular location: involved in miRNA processing in the nucleus and acts as a tRNA pseudouridylate synthase in the cytoplasm. In the cytoplasm, acts as a pseudouridylate synthase by catalyzing synthesis of pseudouridine(54) and pseudouridine(55) from uracil-54 and uracil-55, respectively, in the psi GC loop of a subset of tRNAs. tRNA pseudouridylate synthase activity is enhanced by the presence of 1-methyladenosine at position 53-61 of tRNAs. Does not show tRNA pseudouridylate synthase activity in the nucleus. In the nucleus, promotes primary microRNAs (pri-miRNAs) processing independently of its RNA pseudouridylate synthase activity. Binds pri-miRNAs. Modulator of TRAIL/TNFSF10-induced cell death via activation of procaspase-8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death. {ECO:0000250|UniProtKB:Q3MIT2}.

Molecular Weight: 59.7 kDa

UniProt: Q9D3U0

AlphaFold: Q9D3U0