MEIOB Protein (AA 1-470) (His tag)

Catalog No. ABIN7561002

Recombinant MEIOB protein expressed in HEK-293 Cells.

Quick Overview for MEIOB Protein (AA 1-470) (His tag) (ABIN7561002)

Target: MEIOB (Meiosis Specific with OB Domains (MEIOB))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-470

Purification tag / Conjugate: This MEIOB protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Meiob Protein expressed in mammalian cells.

Sequence: MAKFFALKNF TALSDLHPNM ANLKIIGIVI GKTDVKGFPD RKNIGSERYT FSFTIRDSPC HFVNVSSWGS EDYIRSLSES FRVAECVIIE NPLIQRKDTE REEKFSPATP SNYKLLLSEN HSMVKVCSPY EVDTKLLSLI HLPVKESRDY YSLADIVANG HSLDGRIINV LAAVRSVGEP KYFTTSDRRK GQRCEVKLFD ETEPSFTMTC WDNESILLAQ SWMARETVIF ASDVRINFNK FQNCMAATVI SKTIITVNPD TPEANILLNY IRENKETNVA DEIDSYLKES VNLNTIVDVY TVEQLKVKAL KSEGKADPFY GILYAYISTL NIDDETTKVV RNRCSSCGYI VNEASNTCTI CNQDSSRLKS FFLSFDVLVD LTDHTGTLHS CSLSGSIAEE TLGCTINEFL TMTSEQKTKL KWQLLLERSK IYLKLILSHR ARGGLKVTIL SCKLADPTEA SRNLARQGHT Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: MEIOB (Meiosis Specific with OB Domains (MEIOB))

Alternative Name: Meiob

Background: Meiosis-specific with OB domain-containing protein (EC 3.1.-.-) (Protein expressed in male leptotene and zygotene spermatocytes 675) (MLZ-675),FUNCTION: Single-stranded DNA-binding protein required for homologous recombination in meiosis I. Required for double strand breaks (DSBs) repair and crossover formation and promotion of faithful and complete synapsis. Not required for the initial loading of recombinases but required to maintain a proper number of RAD51 and DMC1 foci after the zygotene stage. May act by ensuring the stabilization of recombinases, which is required for successful homology search and meiotic recombination. Displays Single-stranded DNA 3'-5' exonuclease activity in vitro. {ECO:0000269|PubMed:20339383, ECO:0000269|PubMed:24068956, ECO:0000269|PubMed:24240703}.

Molecular Weight: 53.0 kDa

UniProt: Q9D513

AlphaFold: Q9D513