GID8/C20orf11 Protein (AA 1-228) (His tag)

Catalog No. ABIN7561089

Recombinant GID8/C20orf11 protein expressed in HEK-293 Cells.

Quick Overview for GID8/C20orf11 Protein (AA 1-228) (His tag) (ABIN7561089)

Target: GID8/C20orf11 (GID8) (GID Complex Subunit 8 Homolog (GID8))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-228

Purification tag / Conjugate: This GID8/C20orf11 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Gid8 Protein expressed in mammalian cells.

Sequence: MSYAEKPDEI TKDEWMEKLN NLHVQRADMN RLIMNYLVTE GFKEAAEKFR MESGIEPSVD LETLDERIKI REMILKGQIQ EAIALINSLH PELLDTNRYL YFHLQQQHLI ELIRQRETEA ALEFAQTQLA EQGEESRECL TEMERTLALL AFDSPEESPF GDLLHMMQRQ KVWSEVNQAV LDYENRESTP KLAKLLKLLL WAQNELDQKK VKYPKMTDLS KGVIEEPK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: GID8/C20orf11 (GID8) (GID Complex Subunit 8 Homolog (GID8))

Alternative Name: Gid8

Background: Glucose-induced degradation protein 8 homolog (Two hybrid-associated protein 1 with RanBPM) (Twa1),FUNCTION: Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. Acts as a positive regulator of Wnt signaling pathway by promoting beta-catenin (CTNNB1) nuclear accumulation. {ECO:0000250|UniProtKB:Q9NWU2}.

Molecular Weight: 26.8 kDa

UniProt: Q9D7M1

AlphaFold: Q9D7M1