LITAF Protein (AA 1-161) (His tag)

Catalog No. ABIN7561677

Recombinant LITAF protein expressed in HEK-293 Cells.

Quick Overview for LITAF Protein (AA 1-161) (His tag) (ABIN7561677)

Target: LITAF (Lipopolysaccharide-Induced Tumor Necrosis Factor-alpha Factor (LITAF))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-161

Purification tag / Conjugate: This LITAF protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Litaf Protein expressed in mammalian cells.

Sequence: MSAPGPYQAA AGPSVVPTAP PTYEETVGVN SYYPTPPAPM PGPATGLITG PDGKGMNPPS YYTQPVPVPN ANAIAVQTVY VQQPVSFYDR PVQMCCPSCS KMIVTQLSYN AGALTWLSCG SLCLLGCVAG CCFIPFCVDA LQDVDHYCPN CKALLGTYKR L Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: LITAF (Lipopolysaccharide-Induced Tumor Necrosis Factor-alpha Factor (LITAF))

Alternative Name: Litaf

Background: Lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog (LPS-induced TNF-alpha factor homolog) (Estrogen-enhanced transcript protein) (mEET) (LITAF-like protein) (NEDD4 WW domain-binding protein 3),FUNCTION: Plays a role in endosomal protein trafficking and in targeting proteins for lysosomal degradation. Plays a role in targeting endocytosed EGFR and ERGG3 for lysosomal degradation, and thereby helps down-regulate downstream signaling cascades (PubMed:23166352). Helps recruit the ESCRT complex components TSG101, HGS and STAM to cytoplasmic membranes. Probably plays a role in regulating protein degradation via its interaction with NEDD4 (By similarity). May also contribute to the regulation of gene expression in the nucleus. Binds DNA (in vitro) and may play a synergistic role with STAT6 in the nucleus in regulating the expression of various cytokines (PubMed:15793005, PubMed:21980379). May regulate the expression of numerous cytokines, such as TNF, CCL2, CCL5, CXCL1, IL1A and IL10 (PubMed:12355436, PubMed:15025820, PubMed:16954198, PubMed:21980379, PubMed:22160695). {ECO:0000250|UniProtKB:Q99732, ECO:0000269|PubMed:12355436, ECO:0000269|PubMed:15025820, ECO:0000269|PubMed:15793005, ECO:0000269|PubMed:16954198, ECO:0000269|PubMed:21980379, ECO:0000269|PubMed:23166352}.

Molecular Weight: 16.9 kDa

UniProt: Q9JLJ0

AlphaFold: Q9JLJ0

Pathways: Cellular Response to Molecule of Bacterial Origin