FEN1 Protein (AA 1-378) (His tag)

Catalog No. ABIN7563210

Recombinant FEN1 protein expressed in HEK-293 Cells.

Quick Overview for FEN1 Protein (AA 1-378) (His tag) (ABIN7563210)

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-378

Purification tag / Conjugate: This FEN1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Fen1 Protein expressed in mammalian cells.

Sequence: MGIHGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET TSLMGMFYRT IRMENGIKPV YVFDGKPPQL KSGELAKRSE RRAEAEKQLQ QAQEAGMEEE VEKFTKRLVK VTKQHNDECK HLLSLMGIPY LDAPSEAEAS CAALAKAGKV YAAATEDMDC LTFGSPVLMR HLTASEAKKL PIQEFHLSRV LQELGLNQEQ FVDLCILLGS DYCESIRGIG AKRAVDLIQK HKSIEEIVRR LDPSKYPVPE NWLHKEAQQL FLEPEVVDPE SVELKWSEPN EEELVKFMCG EKQFSEERIR SGVKRLSKSR QGSTQGRLDD FFKVTGSLSS AKRKEPEPKG PAKKKAKTGG AGKFRRGK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Alternative Name: Fen1

Background: Flap endonuclease 1 (FEN-1) (EC 3.1.-.-) (Flap structure-specific endonuclease 1),FUNCTION: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. {ECO:0000255|HAMAP-Rule:MF_03140, ECO:0000269|PubMed:7926735}.

Molecular Weight: 42.3 kDa

UniProt: P39749

AlphaFold: P39749

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA