AGBL4 Protein (AA 1-540) (His tag)

Catalog No. ABIN7563615

Recombinant AGBL4 protein expressed in HEK-293 Cells.

Quick Overview for AGBL4 Protein (AA 1-540) (His tag) (ABIN7563615)

Target: AGBL4 (ATP/GTP Binding Protein-Like 4 (AGBL4))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-540

Purification tag / Conjugate: This AGBL4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Agbl4 Protein expressed in mammalian cells.

Sequence: MAERSQTAPE AGNDTGNEDA IGGNVNKYIV LPNGYSGQPK KGHLTFDACF ESGNLGRVEQ VSDFEYDLFI RPDTCNPRFR VWFNFTVENV KELQRVIFNI VNFSKTKSLY RDGMAPMVKS TSRPKWQRLP PKNVYYYRCP DHRKNYVMSF AFCFDREDDI YQFAYCYPYT YTRFQHYLDS LQKKNMDYFF REQLGQSVQQ RQLDLLTITS PENLREGSEK KVIFITGRVH PGETPSSFVC QGIIDFLVSQ HPIARVLREH LVFKIAPMLN PDGVYLGNYR CSLMGFDLNR HWLDPSPWAH PTLHGVKQLI IKMYNDPKTS LEFYIDIHAH STMMNGFMYG NIFEDEERFQ RQSIFPKLLC QNAEDFSYTS TSFNRDAVKA GTGRRFLGGL LDHSSYCYTL EVSFYSYIIG GTTAAVPYTE EAYMKLGRNV ARTFLDYYRL NSLVEKIAVP MPRLRSKEER RLGWEHPSCL RAEQPLEVLG IPMCSGKALN EHLGNDIQWH LDCGSSTLPL GLISCSPSSS ASWNDMAMSN SILLPDHSFH Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: AGBL4 (ATP/GTP Binding Protein-Like 4 (AGBL4))

Alternative Name: Agbl4

Background: Cytosolic carboxypeptidase 6 (EC 3.4.17.-) (EC 3.4.17.24) (ATP/GTP-binding protein-like 4) (Protein deglutamylase CCP6),FUNCTION: Metallocarboxypeptidase that mediates protein deglutamylation of tubulin and non-tubulin target proteins (PubMed:21074048, PubMed:25103237, PubMed:26829768, PubMed:29593216). Catalyzes the removal of polyglutamate side chains present on the gamma-carboxyl group of glutamate residues within the C-terminal tail of tubulin protein (PubMed:21074048, PubMed:25103237). Specifically cleaves tubulin long-side-chains, while it is not able to remove the branching point glutamate (PubMed:25103237). Also catalyzes the removal of polyglutamate residues from the carboxy-terminus of non-tubulin proteins such as MYLK (PubMed:21074048). Mediates the deglutamylation of nucleotidyltransferase CGAS, leading to CGAS antiviral defense response activation (PubMed:26829768). Involved in KLF4 deglutamylation which promotes KLF4 proteasome-mediated degradation, thereby negatively regulating cell pluripotency maintenance and embryogenesis (PubMed:29593216). {ECO:0000269|PubMed:21074048, ECO:0000269|PubMed:25103237, ECO:0000269|PubMed:26829768, ECO:0000269|PubMed:29593216}.

Molecular Weight: 62.2 kDa

UniProt: Q09LZ8

AlphaFold: Q09LZ8