CTC1 Protein (AA 1-1212) (His tag)

Catalog No. ABIN7563838

This Recombinant CTC1 protein is produced in HEK-293 Cells.

Quick Overview for CTC1 Protein (AA 1-1212) (His tag) (ABIN7563838)

Target: CTC1 (CTS Telomere Maintenance Complex Component 1 (CTC1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1212

Purification tag / Conjugate: This CTC1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Ctc1 Protein expressed in mammalian cells.

Sequence: MAACRAQPPT SEQAWLEAAQ TFIQETLCPA GKEVDKELTR SVIACVKETW LSQGENQDLT LPFSYSFVSV QSLKTHQRLP CCSHLSWSQS AYQAWTRGGR PGDGVLPREQ LILLGTLVDL LGDSEQECRS GSLYVRDNTG TLDCELIDLD LSWLGHLFLF PSWSYLPSAK RNSLGEGHLE LWGTPVPVFP LTVSPGPLIP IPVLYPEKAS HLLRYRKKSS IKEINLAGKL VHLSALIITQ NKRYFIMTLG ELAQAGSQVS IIVQIPAQMV WHRVLRPGRA YVLTKLQVTK TRIHLSCIWT TIPSSTLKPL RPGYVQELEL DLEFSKADLK PPPQPTSSKD SRGQEGLVRA SKVLHYLGTV TAVLHESAGL YILDGQLILC LAYQKIHGLR RVIRPGVCLE LRDVHLLQAV GGATTKPVLA LCLHGTVRLQ GFSCLKPLTL PSSKVYGASL YEQLVWKCQL GLPLYLWAAK TLEDLIYKLC PHVLRCHQFL KQPSPGKPSL GLQLLAPSWD VLIPPGSPMR HAYSEILEEP HNCPLQKYTP LQTPYSFPTM LALAEEGQHR AWATFDPKAM LPLPEASHLT SCQLNRHLAW SWVCLPSCVF QPAQVLLGVL VASSRKGCLE LRDLRGSLPC IPLTESSQPL IDPNLVGCLV RVEKFQLVVE REVRSSFPSW EEMGMARFIQ KKQARVYVQF YLADALILPV PRPTFGSEPS QTASSCPEGP HLGQSRLFLL SHKEALMKRN FCLLPGDSSQ PAKPTLSFHV SGTWLCGTQR KEGSGWSPPE SLAVESKDQK VFLIFLGSSV RWFPFLYPNQ VYRLVASGPS QTPVFETEGS AGTSRRPLEL ADCGSCLTVQ EEWTLELGSS QDIPNVLEVP RTLPESSLAQ LLGDNSPDSL VSFSAEILSR ILCEPPLALR RMKPGNAGAI KTGVKLTVAL EMDDCEYPPH LDIYIEDPQL PPQIGLLPGA RVHFSQLEKR ISRSNIVYCC FRSSTSVQVL SFPPETKASA PLPHIYLAEL LQGDRPPFQA TTSCHIVYVL SLQILWVCAH CTSICPQGKC SRRDPSCPSQ RAVSQANIRL LVEDGTAEAT VICRNHLVAR ALGLSPSEWS SILEHARGPG RVALQFTGLG GQTESASKTH EPLTLLLRTL CTSPFVLRPV KLSFALERRP TDISPREPSR LQQFQCGELP LLTRVNPRLR LVCLSLQEPE LPNPPQASAA SS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: CTC1 (CTS Telomere Maintenance Complex Component 1 (CTC1))

Alternative Name: Ctc1

Background: CST complex subunit CTC1 (Alpha-accessory factor of 132 kDa) (AAF-132) (AAF132) (Conserved telomere maintenance component 1),FUNCTION: Component of the CST complex proposed to act as a specialized replication factor promoting DNA replication under conditions of replication stress or natural replication barriers such as the telomere duplex. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. Initially the CST complex has been proposed to protect telomeres from DNA degradation (PubMed:19854130). However, the CST complex has been shown to be involved in several aspects of telomere replication. The CST complex inhibits telomerase and is involved in telomere length homeostasis, it is proposed to bind to newly telomerase-synthesized 3' overhangs and to terminate telomerase action implicating the association with the ACD:POT1 complex thus interfering with its telomerase stimulation activity. The CST complex is also proposed to be involved in fill-in synthesis of the telomeric C-strand probably implicating recruitment and activation of DNA polymerase alpha. The CST complex facilitates recovery from many forms of exogenous DNA damage, seems to be involved in the re-initiation of DNA replication at repaired forks and/or dormant origins. Involved in telomere maintenance. Involved in genome stability (By similarity). May be in involved in telomeric C-strand fill-in during late S/G2 phase (PubMed:22748632). {ECO:0000250|UniProtKB:Q2NKJ3, ECO:0000269|PubMed:19854130, ECO:0000269|PubMed:22748632}.

Molecular Weight: 134.0 kDa

UniProt: Q5SUQ9

AlphaFold: Q5SUQ9