KLF4 Protein (AA 1-483) (His tag)

Catalog No. ABIN7563877

This Recombinant KLF4 protein is produced in HEK-293 Cells.

Quick Overview for KLF4 Protein (AA 1-483) (His tag) (ABIN7563877)

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-483

Purification tag / Conjugate: This KLF4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Klf4 Protein expressed in mammalian cells.

Sequence: MRQPPGESDM AVSDALLPSF STFASGPAGR EKTLRPAGAP TNRWREELSH MKRLPPLPGR PYDLAATVAT DLESGGAGAA CSSNNPALLA RRETEEFNDL LDLDFILSNS LTHQESVAAT VTTSASASSS SSPASSGPAS APSTCSFSYP IRAGGDPGVA ASNTGGGLLY SRESAPPPTA PFNLADINDV SPSGGFVAEL LRPELDPVYI PPQQPQPPGG GLMGKFVLKA SLTTPGSEYS SPSVISVSKG SPDGSHPVVV APYSGGPPRM CPKIKQEAVP SCTVSRSLEA HLSAGPQLSN GHRPNTHDFP LGRQLPTRTT PTLSPEELLN SRDCHPGLPL PPGFHPHPGP NYPPFLPDQM QSQVPSLHYQ ELMPPGSCLP EEPKPKRGRR SWPRKRTATH TCDYAGCGKT YTKSSHLKAH LRTHTGEKPY HCDWDGCGWK FARSDELTRH YRKHTGHRPF QCQKCDRAFS RSDHLALHMK RHF Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Alternative Name: Klf4

Background: Krueppel-like factor 4 (Epithelial zinc finger protein EZF) (Gut-enriched krueppel-like factor),FUNCTION: Transcription factor, can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence (PubMed:10431239, PubMed:10556311, PubMed:15358627, PubMed:16954384, PubMed:17060454, PubMed:19816951, PubMed:20071344, PubMed:29593216). Binds to the promoter region of its own gene and can activate its own transcription (PubMed:10431239, PubMed:10556311, PubMed:15358627, PubMed:16954384, PubMed:17060454, PubMed:19816951, PubMed:20071344, PubMed:29593216). Regulates the expression of key transcription factors during embryonic development (PubMed:10431239, PubMed:10556311, PubMed:15358627, PubMed:16954384, PubMed:17060454, PubMed:19816951, PubMed:20071344, PubMed:29593216). Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation (PubMed:10431239, PubMed:10556311, PubMed:15358627, PubMed:16954384, PubMed:17060454, PubMed:19816951, PubMed:20071344, PubMed:29593216). Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription (By similarity). {ECO:0000250, ECO:0000269|PubMed:10431239, ECO:0000269|PubMed:10556311, ECO:0000269|PubMed:15358627, ECO:0000269|PubMed:16954384, ECO:0000269|PubMed:17060454, ECO:0000269|PubMed:19816951, ECO:0000269|PubMed:20071344, ECO:0000269|PubMed:29593216}.

Molecular Weight: 51.9 kDa

UniProt: Q60793

AlphaFold: Q60793

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance