Lipin 1 Protein (LPIN1) (AA 1-924) (His tag)

Catalog No. ABIN7564833

Product Details

Target: Lipin 1 (LPIN1)

Protein Type: Recombinant

Protein Characteristics: AA 1-924

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This Lipin 1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Lpin1 Protein expressed in mammalian cells.

Sequence: MNYVGQLAGQ VFVTVKELYK GLNPATLSGC IDIIVIRQPN GSLQCSPFHV RFGKMGVLRS REKVVDIEIN GESVDLHMKL GDNGEAFFVQ ETDNDQEIIP MYLATSPILS EGAARMESQL KRNSVDRIRC LDPTTAAQGL PPSDTPSTGS LGKKRRKRRR KAQLDNLKRD DNVNSSEDED MFPIEMSSDE DTAPMDGSRT LPNDVPPFQD DIPKENFPSI STHPQSASYP SSDREWSPSP SSLVDCQRTP PHLAEGVLSS SCPLQSCHFH ASESPSGSRP STPKSDSELV SKSADRLTPK NNLEMLWLWG ELPQAAKSSS PHKMKESSPL GSRKTPDKMN FQAIHSESSD TFSDQSPTMA RGLLIHQSKA QTEMQFVNEE DLESLGAAAP PSPVAEELKA PYPNTAQSSS KTDSPSRKKD KRSRHLGADG VYLDDLTDMD PEVAALYFPK NGDPGGLPKQ ASDNGARSAN QSPQSVGGSG IDSGVESTSD SLRDLPSIAI SLCGGLSDHR EITKDAFLEQ AVSYQQFADN PAIIDDPNLV VKVGNKYYNW TTAAPLLLAM QAFQKPLPKA TVESIMRDKM PKKGGRWWFS WRGRNATIKE ESKPEQCLTG KGHNTGEQPA QLGLATRIKH ESSSSDEEHA AAKPSGSSHL SLLSNVSYKK TLRLTSEQLK SLKLKNGPND VVFSVTTQYQ GTCRCEGTIY LWNWDDKVII SDIDGTITRS DTLGHILPTL GKDWTHQGIA KLYHKVSQNG YKFLYCSARA IGMADMTRGY LHWVNERGTV LPQGPLLLSP SSLFSALHRE VIEKKPEKFK VQCLTDIKNL FFPNTEPFYA AFGNRPADVY SYKQVGVSLN RIFTVNPKGE LVQEHAKTNI SSYVRLCEVV DHVFPLLKRS HSCDFPCSDT FSNFTFWREP LPPFENQDMH SASA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Lipin 1 (LPIN1)

Alternative Name: Lpin1 (LPIN1 Products)

Background: Phosphatidate phosphatase LPIN1 (EC 3.1.3.4) (Fatty liver dystrophy protein) (Lipin-1),FUNCTION: Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis and therefore controls the metabolism of fatty acids at different levels (PubMed:17158099). Acts also as nuclear transcriptional coactivator for PPARGC1A/PPARA regulatory pathway to modulate lipid metabolism gene expression (PubMed:16950137). Is involved in adipocyte differentiation (PubMed:16049017). {ECO:0000269|PubMed:16049017, ECO:0000269|PubMed:16950137, ECO:0000269|PubMed:17158099}., FUNCTION: [Isoform 1]: Recruited at the mitochondrion outer membrane and is involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol. {ECO:0000269|PubMed:21397848}.

Molecular Weight: 102.0 kDa

UniProt: Q91ZP3

AlphaFold: Q91ZP3

Pathways: Monocarboxylic Acid Catabolic Process