ORC3 Protein (AA 1-715) (His tag)

Catalog No. ABIN7565108

This Recombinant ORC3 protein is expressed in HEK-293 Cells.

Quick Overview for ORC3 Protein (AA 1-715) (His tag) (ABIN7565108)

Target: ORC3 (Origin Recognition Complex, Subunit 3 (ORC3))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-715

Purification tag / Conjugate: This ORC3 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Orc3 Protein expressed in mammalian cells.

Sequence: MHTGPRTMAT SSVSKGCFVF KPDFKKRKVF VPIEDYFNNE ELDSEDSKLR FETYSLLWQR MKSETEQLQE ELNENLFDNL VDFLQKSHPE FQKNSRDWGS QMKFREIPTA ALILGVNVTD HDVILRSLTE TLQNNVTPYV VSLQAKDCPD VKHFLQKFTS QLMDCCVDRH SKEVTSGKAL KKTNYSMDSL CSWYSAVTQK ADHKVTIKKR TASGHWRSPP VVLILKSMES FTSKVLQDFI TISSQHLHEF PLILIFGIAT SPVIIHRLLP HSVSSLLCVE LFQSLSCEQH LTVVLDKLLL TPQFPFKLSK KALQVLTNIF LYHDFSIQSF IKGIKLSLLE HFYSQPLSVL CCDLSEAKKR VNVFSVSQCE NIRRLPSFRR YVENQPLGKQ VALLTNETFL KEKTQSLLED LHVYHINYFL VLRCLHNFTS SLPKYPLGRQ IRELYCTCLE KKIWDSEEYK SALQLLRMLA KDELVSILQR CIEVLDSSTE KQLGNTTQKI KDFLTQFQNL DADSKEEEDA CGSQPKGLQK TDLYHLQKSL LEMKELRRTK KPTKFEMLRE NVMNFIDNLV RDYLLPPESQ PLHEVVYFSA ANTLREHLNA APRIALHTAL NNPYYYLKNE ELEGCIPNTA PDICIAYKLH LECSLLINLV DWAEAFATVV TAAEKMDANS TVSEEMSEVI HARFIRAVSE LELLGFIKPT KQKTDHVARL TWGGC Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ORC3 (Origin Recognition Complex, Subunit 3 (ORC3))

Alternative Name: Orc3

Background: Origin recognition complex subunit 3 (Origin recognition complex subunit Latheo),FUNCTION: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3. {ECO:0000250|UniProtKB:Q9UBD5}.

Molecular Weight: 82.3 kDa

UniProt: Q9JK30

AlphaFold: Q9JK30

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Feeding Behaviour, Synthesis of DNA