DPF1 Protein (AA 1-387) (His tag)

Catalog No. ABIN7565142

Recombinant DPF1 protein expressed in HEK-293 Cells.

Quick Overview for DPF1 Protein (AA 1-387) (His tag) (ABIN7565142)

Target: DPF1 (D4, Zinc and Double PHD Fingers Family 1 (DPF1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-387

Purification tag / Conjugate: This DPF1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Dpf1 Protein expressed in mammalian cells.

Sequence: MATVIPSPLS LGEDFYREAI EHCRSYNARL CAERSLRLPF LDSQTGVAQN NCYIWMEKTH RGPGLAPGQI YTYPARCWRK KRRLNILEDP RLRPCEYKID CEAPLKKEGG LPEGPVLEAL LCAETGEKKV ELKEEETIMD CQKQQLLEFP HDLEVEDLEE DIPRRKNRAR GKAYGIGGLR KRQDTASLED RDKPYVCDIC GKRYKNRPGL SYHYTHTHLA EEEGEEHTER HALPFHRKNN HKQFYKELAW VPEAQRKHTA KKAPDGTVIP NGYCDFCLGG SKKTGCPEDL ISCADCGRSG HPSCLQFTVN MTAAVRTYRW QCIECKSCSL CGTSENDDQL LFCDDCDRGY HMYCLSPPMA EPPEGSWSCH LCLRHLKEKA SAYITLT Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DPF1 (D4, Zinc and Double PHD Fingers Family 1 (DPF1))

Alternative Name: Dpf1

Background: Zinc finger protein neuro-d4 (BRG1-associated factor 45B) (BAF45B) (D4, zinc and double PHD fingers family 1),FUNCTION: May have an important role in developing neurons by participating in regulation of cell survival, possibly as a neurospecific transcription factor. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. {ECO:0000269|PubMed:17640523}.

Molecular Weight: 44.2 kDa

UniProt: Q9QX66

AlphaFold: Q9QX66