IL1A Protein (AA 113-271)

Catalog No. ABIN7566200

This Recombinant IL1A protein is produced in HEK-293 Cells.

Quick Overview for IL1A Protein (AA 113-271) (ABIN7566200)

Target: IL1A (Interleukin 1 alpha (IL1A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 113-271

Purpose: IL-1alpha (human) (rec.)

Cross-Reactivity: Human

Characteristics: Human IL-1alpha (aa 113-271).

Sterility: Sterile filtered

Endotoxin Level: <0.01EU/μg protein (LAL test, Lonza).

Biological Activity Comment: The ED50 as determined by the dose-dependent stimulation of murine D10S cells is < 0.001 ng/ml, corresponding to a specific activity of > 1 x 109units/mg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL-1alpha

Background:

Interleukin-1alpha, IL-1F1, IL-1A

The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1alpha and IL-1beta. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1alpha was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1alpha upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1alpha is not mediated by caspase-1 and is not required for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1alpha and IL-1beta. Although most activators fully rely on the inflammasome for IL-1alpha secretion, some induce the processing and secretion of IL-1alpha in an inflammasome-independent manner.

Molecular Weight: ~19kDa (SDS-PAGE)

NCBI Accession: NP_000566

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints