IL-21 Protein (AA 23-155)

Catalog No. ABIN7566234

This Recombinant IL-21 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-21 Protein (AA 23-155) (ABIN7566234)

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 23-155

Purpose: IL-21 (human) (rec.) (untagged)

Cross-Reactivity: Human

Characteristics: Human IL-21 (aa 23-155) with a N-terminal Met.

Sterility: Sterile filtered

Endotoxin Level: <0.01EU/μg protein (LAL test, Lonza).

Biological Activity Comment: Measured by its ability to induce interferon-gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.4-2ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -80°C

Use & Stability: Stable for at least 1 year after receipt when stored at -80°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Alternative Name: IL-21

Background:

Interleukin-21

Interleukin-21 (IL-21) is a key factor in the transition between innate and adaptive immune responses secreted by activated T cells. The IL-21 receptor (IL-21R) is expressed in lymphoid tissue, in particular by NK, B, T and dendritic cells, macrophages and endothelial cells. Recent evidence suggests that IL-21 plays a supportive role in the proliferation of T and B cells and influences the cytolytic activity of natural killer cells. IL-21 has been shown to up-regulate genes associated with innate immunity and to inhibit the differentiation of naive T helper cells. IL-21 specifically inhibits IFN-gamma production from developing TH1 cells and is preferentially expressed by TH2 cells. Furthermore IL-21 has been identified as a growth and survival factor for human myeloma cells. IL-21/IL-21R interactions have a unique role in sequentially activating both innate and adaptive immune responses against poorly immunogenic tumors, leading to tumor rejection that is perforin dependent but IFN-gamma independent.

Molecular Weight: ~17kDa (SDS-PAGE)

UniProt: Q9HBE4-1

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response