IL-8 Protein (AA 23-99) (Fc Tag)

Catalog No. ABIN7566311

This Recombinant IL-8 protein is produced in CHO Cells.

Quick Overview for IL-8 Protein (AA 23-99) (Fc Tag) (ABIN7566311)

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: CHO Cells

Purity: >98 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 23-99

Purification tag / Conjugate: This IL-8 protein is labelled with Fc Tag.

Purpose: IL-8 (human):Fc (human) (rec.) (non-lytic)

Cross-Reactivity: Human

Characteristics: The extracellular domain of human IL-8 (aa 23-99) is fused to the N-terminus of the Fc region of a mutant human IgG1.

Sterility: Sterile filtered

Endotoxin Level: <0.06EU/μg protein (LAL test, Lonza).

Biological Activity Comment: Shows the biological function of the IL-8 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: IL-8

Background:

Interleukin-8, CXCL8, C-X-C Motif Chemokine 8, Emoctakin, GCP-1, LAI, MDNCF, MONAP, NAP-1, NCF, TCF

Interleukin-8 (IL-8) was originally discovered as a neutrophil chemotactic and activating factor and is a member of the alpha (CXC) subfamily of chemokines (including also platelet factor 4, GRO, IP-10, etc.). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes and various tumor cell lines, produce IL-8 in response to a wide variety of proinflammatory stimuli such as exposure to IL-1, TNF, LPS and viruses. IL-8 has a wide range of other proinflammatory effects. It is a potent chemoattractant for neutrophils and causes degranulation of neutrophil specific granules and azurophilic granules. IL-8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and subendothelial matrix proteins. Besides neutrophils, IL-8 is also chemotactic for basophils, T cells and eosinophils. IL-8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. IL-8 was also reported to be angiogenic both in vivo and in vitro.

NCBI Accession: NP_000575

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy