alpha-Bungarotoxin (Active) protein (Atto 550)

Catalog No. ABIN7582314

This Native alpha-Bungarotoxin protein is expressed in Bungarus multicinctus (Many-banded krait).

Quick Overview for alpha-Bungarotoxin (Active) protein (Atto 550) (ABIN7582314)

Target: alpha-Bungarotoxin

Protein Type: Native

Biological Activity: Active

Origin: Bungarus multicinctus

Source: Bungarus multicinctus (Many-banded krait)

Application: Fluorescence Microscopy (FM), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Product Details

Purification tag / Conjugate: Atto 550

Purpose: α-Bungarotoxin-ATTO Fluor-550

Sequence: IVCHTTATSP ISAVTCPPGE NLCYRKMWCD AFCSSRGKVV ELGCAATCPS KKPYEEVTCC STDKCNPHPK QRPG

Specificity: Toxin target: α1/β1/γ/δ, α7 nAChR, GABA(A) receptor subtypes

Characteristics: ATTO 550 (Ex/Em: 554/576 nm). The extent of labeling is 1-3 Molecules of dye per molecule α-Bungarotoxin, linked via primary amines.

Biological Activity Comment: α-Bungarotoxin blocks postsynaptic neuromuscular transmission via competitive inhibition of nicotinic ACh receptors (nAChRs).

Application Details

Application Notes: Antigen Preadsorption Control: 0.1 - 0.5 μM

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Note: The product is typically supplied in a 0.5 mL conical vial. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles and exposure to light to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Handling Advice: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid vigorous vortexing. protect from moisture,keep the vial protected from light

Storage: -20 °C

Storage Comment: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C, protect from moisture, and keep the vial protected from light.

Target Details

Target: alpha-Bungarotoxin

Background: A fluorescent probe for imaging and sensitive detection of neuromuscular junctions, GABA(A) receptors, nAChRs, and α-Bungarotoxin binding sites

Molecular Weight: ~9134 Da