Dendrotoxin-I (Active) Protein

Catalog No. ABIN7887260

This Native Dendrotoxin-I protein is produced in Dendroaspis polylepis polylepis (Black mamba snake).

Quick Overview for Dendrotoxin-I (Active) Protein (ABIN7887260)

Target: Dendrotoxin-I

Protein Type: Native

Biological Activity: Active

Origin: Dendroaspis polylepis

Source: Dendroaspis polylepis polylepis (Black mamba snake)

Purity: ≥98 % (HPLC)

Product Details

Purpose: Dendrotoxin-I

Sequence: QPLRKLCILH RNPGRCYQKI PAFYYNQKKK QCEGFTWSGC GGNSNRFKTI EECRRTCIRK -OH

Specificity: Toxin target: KV1.1, KV1.2, KV1.6 voltage-gated potassium channels

Biological Activity Comment: Dendrotoxin-I is a highly selective blocker of voltage-gated K+ channels (KV1.1 and KV1.2), as well as heteromultimeric channels containing these, with other KV1 isoforms1. The inhibition effect is higher in Xenopus oocytes compared to mammalian cells2.

Application Details

Application Notes: Antigen Preadsorption Control: 10 - 100 nM

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 100 μM to 1  mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Handling Advice: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture

Storage: -20 °C

Storage Comment: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Target Details

Target: Dendrotoxin-I

Background: A potent and selective blocker of neuronal KV1.1, KV1.2, KV1.6 potassium channels

Molecular Weight: 7149 Da

UniProt: P00979

CAS-No: 107950-33-4