alpha-Latrotoxin (Active) Protein

Catalog No. ABIN7887287

This Native alpha-Latrotoxin protein is expressed in Latrodectus tredecimguttatus (Mediterranean black widow spider).

Quick Overview for alpha-Latrotoxin (Active) Protein (ABIN7887287)

Target: alpha-Latrotoxin

Protein Type: Native

Biological Activity: Active

Origin: Latrodectus tredecimguttatus

Source: Latrodectus tredecimguttatus (Mediterranean black widow spider)

Purity: ≥97 %

Product Details

Purpose: α-Latrotoxin

Specificity: Toxin target: Neurexin-1, ADGRL1 (latrophilin-1/CIRL), and tyrosine-protein phosphatase S (PTPRS) receptors

Biological Activity Comment: α-Latrotoxin induces exhaustive neurotransmitter release from nerve terminals1. Indirectly activates the ERK MAP kinase pathway.

Application Details

Application Notes: Antigen Preadsorption Control: 150 pM - 1 nM

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Carefully add the solvent directly to the vial interior. Dissolve the material by gently tapping, tilting, or rolling the vial. Avoid vigorous shaking or vortexing Reconstitute in high-quality pure water or a conventional buffer (e.g., 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.1 mM CaCl₂, and 0.2 mM MgCl₂). Avoid multiple freeze-thaw cycles and maintain optimal biological activity.

Buffer: Lyophilized from 20 mM KPO4 buffer, pH 6.8.

Handling Advice: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. protect from moisture

Storage: -20 °C

Storage Comment: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Target Details

Target: alpha-Latrotoxin

Background: A potent inducer of massive neurotransmitter release via presynaptic neurexin and latrophilin receptors

Molecular Weight: 130 kDa

UniProt: P23631

CAS-No: 65988-34-3