MitTx Toxin (Active) Protein
Native MitTx Toxin protein expressed in Micrurus tener tener (Texas coral snake).
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Catalog No. ABIN7887288
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Quick Overview for MitTx Toxin (Active) Protein (ABIN7887288)
Target
MitTx Toxin
Protein Type
Native
Biological Activity
Active
Origin
Micrurus tener tener
Source
Micrurus tener tener (Texas coral snake)
Purity
≥98 % (HPLC)
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Purpose
- MitTx Toxin
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Sequence
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MitTx-α subunit:
QIRPAFCYED PPFFQKCGAF VDSYYFNRSR ITCVHFFYGQ CDVNQNHFTT MSECNRVCHG
MitTx-β subunit:
NLNQFRLMIK CTNDRVWADF VDYGCYCVAR DSNTPVDDLD RCCQAQKQCY DEAVKVHGCK PLVMFYSFEC RYLASDLDCS GNNTKCRNFV CNCDRTATLC ILTATYNRNN HKIDPSRCQ -
Specificity
- Toxin target: ASIC1-containing acid-sensing ion channels (ASICs)
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Biological Activity Comment
- MitTx toxin activates ASIC1-containing channels1,2.
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Application Notes
- Antigen Preadsorption Control: 20 nM
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Comment
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Electrophysiology
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 100 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.
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Buffer
- Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).
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Handling Advice
- Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture
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Storage
- -20 °C
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Storage Comment
- The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.
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