Muscarinic Toxin 3 (Active) Protein

Catalog No. ABIN7887291

This Native Muscarinic Toxin 3 protein is produced in Dendroaspis angusticeps (Eastern green mamba).

Quick Overview for Muscarinic Toxin 3 (Active) Protein (ABIN7887291)

Target: Muscarinic Toxin 3

Protein Type: Native

Biological Activity: Active

Origin: Dendroaspis angusticeps

Source: Dendroaspis angusticeps (Eastern green mamba)

Purity: >99 % (HPLC)

Product Details

Purpose: Muscarinic Toxin 3

Sequence: LTCVTKNTIF GITTENCPAG QNLCFKRWHY VIPRYTEITR GCAATCPIPE NYDSIHCCKT DKCNE-OH

Specificity: Toxin target: M4 muscarinic, α1A, α2A, α2C, α1B, and α1D adrenoceptors

Biological Activity Comment: Muscarinic receptor 4 and adrenoceptor antagonist1-4.

Application Details

Application Notes: Antigen Preadsorption Control: 100 nM - 1 μM

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 100 μM to 1  mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Handling Advice: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture

Storage: -20 °C

Storage Comment: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Target Details

Target: Muscarinic Toxin 3

Background: A potent antagonist selective for the M4 muscarinic acetylcholine receptor (M4 mAChR) and a non-selective antagonist for several α1- and α2-adrenoceptor subtypes

Molecular Weight: 7379 Da

UniProt: P81031