Tetanus Toxin Protein (TetX)

Catalog No. ABIN7582361

This Native Tetanus Toxin protein is produced in Clostridium tetani.

Quick Overview for Tetanus Toxin Protein (TetX) (ABIN7582361)

Target: Tetanus Toxin (TetX)

Protein Type: Native

Biological Activity: Active

Origin: Clostridium tetani

Source: Clostridium tetani

Application: Cellular Assay (CA), Western Blotting (WB)

Purity: ≥97 % (HPLC)

Product Details

Purpose: Tetanus Toxin

Specificity: Toxin target: VAMP2 (synaptobrevin-2)

Biological Activity Comment: Tetanus Toxin inhibits neurotransmitter release by cleaving synaptobrevin-2 in a zinc-dependent manner1,2.

Application Details

Application Notes: Antigen Preadsorption Control: 1 - 100 nM

Comment:

Electrophysiology

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Carefully add the solvent directly to the vial interior. Dissolve the material by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, gentle vortex of 1-2 seconds may be used if necessary to achieve complete dissolution. Reconstitute in sterile, high-purity water or a conventional buffer (e.g., 0.1 M sodium chloride and 5 % trehalose at pH 7.0). Aliquot the reconstituted solution and store at -20 °C to -80 °C to avoid multiple freeze-thaw cycles and maintain optimal biological activity.

Buffer: Lyophilized Powder.

Handling Advice: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid vigorous vortexing. protect from moisture

Storage: -20 °C

Storage Comment: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Target Details

Target: Tetanus Toxin (TetX)

Alternative Name: Tetanus Toxin

Target Type: Bacteria

Background: An inhibitor of neurotransmitter release

Molecular Weight: 150 kDa

UniProt: P04958

CAS-No: 676570-37-9