GFRA1 Protein (AA 25-424) (Fc Tag)
Quick Overview for GFRA1 Protein (AA 25-424) (Fc Tag) (ABIN7678164)
Target
See all GFRA1 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
-
-
Protein Characteristics
- AA 25-424
-
Purification tag / Conjugate
- This GFRA1 protein is labelled with Fc Tag.
-
Sequence
-
DRLDCVKASD QCLKEQSCST KYRTLRQCVA GKETNFSLAS GLEAKDECRS AMEALKQKSL YNCRCKRGMK KEKNCLRIYW SMYQSLQGND LLEDSPYEPV NSRLSDIFRV VPFISVEHIP KGNNCLDAAK ACNLDDICKK YRSAYITPCT TSVSNDVCNR RKCHKALRQF FDKVPAKHSY GMLFCSCRDI ACTERRRQTI VPVCSYEERE KPNCLNLQDS CKTNYICRSR LADFFTNCQP ESRSVSSCLK ENYADCLLAY SGLIGTVMTP NYIDSSSLSV APWCDCSNSG NDLEECLKFL NFFKDNTCLK NAIQAFGNGS DVTVWQPAFP VQTTTATTTT ALRVKNKPLG PAGSENEIPT HVLPPCANLQ AQKLKSNVSG NTHLCISNGN YEKEGLGASS
-
Purification
- > 90% by SDS-PAGE
-
Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
-
Biological Activity Comment
- Measured by its binding ability in a functional ELISA with Human GDNF (CAT# ATGP3942). The ED50 range ≤ 50 ng/ml.
-
-
Want other Options for this Protein ?
!Discover Our Predefined Custom Proteins and Custom Protein Services!Your project requires further customization? Contact us and discover our custom protein solutions
-
GDNF Family Receptor alpha 1 (GFRA1) (AA 25-424) (Active) protein (His tag)
GFRA1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
GDNF Family Receptor alpha 1 (GFRA1) (AA 1-460) protein (GST tag)GFRA1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
GDNF Family Receptor alpha 1 (GFRA1) (Active) protein (Fc Tag)GFRA1 Origin: Rat Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
GDNF Family Receptor alpha 1 (GFRA1) (Active) protein (His tag)GFRA1 Origin: Mouse Host: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
GDNF Family Receptor alpha 1 (GFRA1) (Active) protein (His tag)GFRA1 Origin: Rat Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
GDNF Family Receptor alpha 1 (GFRA1) (AA 25-430) protein (hIgG-His-tag)GFRA1 Origin: Rat Host: Baculovirus infected Insect Cells Recombinant > 85 % SDS
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Concentration
- 0.5 mg/mL
-
Buffer
- Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol
-
Storage
- 4 °C,-20 °C,-80 °C
-
Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
-
-
- GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))
-
Alternative Name
- GFR alpha-1
-
Background
- GFR alpha-1, also known as GDNF family receptor alpha-1, belongs to the GDNFR family. It is a glycosylphosphatidylinositol (GPI)-linked receptor found on the cell surface, acting as a receptor for both GDNF and NTN and function as neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. It bind GDNF strongly and activate RET tyrosine kinase and is also essential for the development of kidneys and the enteric nervous system. Recombinant human GFR alpha-1, fused to hIgG-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
-
Molecular Weight
- 70.8 kDa (633aa)
-
NCBI Accession
- NP_665736
Target
-
