FGF2 Protein (AA 143-288)

Catalog No. ABIN7596209

This Recombinant FGF2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for FGF2 Protein (AA 143-288) (ABIN7596209)

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Activity Assay (AcA)

Purity: > 95% by SDS-PAGE

Product Details

Protein Characteristics: AA 143-288

Sequence: MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells in the presence of 10 ug/ml of heparin. The ED50 range ≤ 0.2 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Alternative Name: FGF basic/FGF-2

Background: Fibroblast growth factor 2 (FGF2) is a member of the FGF family that binds heparin and plays important roles in morphogenic, neurotrophic and angiogenic processes. FGF2 possess diverse biological functions, such as neuron differentiation, embryonic development and differentiation, modulation of angiogenesis, and wound healing. Recombinant human FGF2 was expressed in E. coli and purified by conventional chromatography techniques.

Molecular Weight: 16.5 kDa (147aa) confirmed by MALDI-TOF

NCBI Accession: NP_001997

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins