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IL-2 Protein (C160S)

This Recombinant IL-2 protein is produced in Escherichia coli (E. coli).
Catalog No. ABIN7596214

Quick Overview for IL-2 Protein (C160S) (ABIN7596214)

Target

See all IL-2 (IL2) Proteins
IL-2 (IL2) (Interleukin 2 (IL2))

Protein Type

Recombinant

Biological Activity

Active

Origin

  • 47
  • 25
  • 9
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Mouse

Source

  • 49
  • 29
  • 23
  • 6
  • 4
  • 3
  • 1
  • 1
  • 1
Escherichia coli (E. coli)

Application

SDS-PAGE (SDS), Activity Assay (AcA)

Purity

> 90% by SDS-PAGE
  • Protein Characteristics

    C160S

    Sequence

    MAPTSSSTSS STAEAQQQQQ QQQQQQQHLE QLLMDLQELL SRMENYRNLK LPRMLTFKFY LPKQATELKD LQCLEDELGP LRHVLDLTQS KSFQLEDAEN FISNIRVTVV KLKGSDNTFE CQFDDESATV VDFLRRWIAF SQSIISTSPQ

    Endotoxin Level

    < 1 EU per 1ug of protein (determined by LAL method)

    Biological Activity Comment

    Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 range ≤0.9ng/ml.
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  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Storage

    4 °C,-20 °C,-80 °C

    Storage Comment

    Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
  • Target

    IL-2 (IL2) (Interleukin 2 (IL2))

    Alternative Name

    IL-2

    Background

    IL2 (Interleukin2), also known as T cell growth factor (TCGF), is an immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant mouse IL2 was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

    Molecular Weight

    17.3 kDa (150aa) confirmed by MALDI-TOF

    NCBI Accession

    NP_032392

    Pathways

    JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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